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Updated: Jul 5, 2025

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

维培林和病毒性出血性败血症病毒之间的反循环调节通过竞争的蛋白质降解途径

Xiaobing Lu^1,2, Meisheng Yi^1,2, Zhe Hu^1,2

¹State Key Laboratory of Biocontrol, Southern Marine Sciences and Engineering Guangdong Laboratory (Zhuhai), School of Marine Sciences, Sun Yat-sen University, Guangzhou, Guangdong, 519082, China.

bioRxiv : the preprint server for biology

|January 23, 2024

概括

维佩林蛋白通过降解病毒蛋白来对抗病毒性出血性败血症病毒 (VHSV). 然而,VHSVVV的

科学领域:

免疫学和病毒学
分子生物学分子生物学

背景情况:

病毒性出血性败血症 (VHS) 是对鱼群的重大威胁,由病毒性出血性败血症病毒 (VHSV) 引起.
缺乏有效的VHS治疗方法,强调需要了解病毒与宿主相互作用以预防疾病.

研究的目的:

为了阐明鱼类中viperin和VHSV之间的复杂的反循环.
为了确定VHSV逃避viperin介导的抗病毒反应的分子机制.
探索预防VHSV感染的潜在治疗点.

主要方法:

研究了 *Lateolabrax japonicus* viperin (Ljviperin) 的诱导途径.
评估了Ljviperin在抗VHSV活性中的C端域的作用.
通过自学分析了VHSV核蛋白 (N) 和蛋白 (P) 的降解.
通过ubiquitin-proteasome通路检查了VHSV N蛋白质对干扰素调节因子 (IRF1) 和IRF9的降解.

主要成果:

利维林通过IFN独立途径和IFN依赖途径诱导.
利维林的C端域对其抗VHSV疗效至关重要.
利维林通过自诱导VHSVN和P蛋白的降解.
VHSV N 蛋白通过无素-蛋白酶体通路降解IRF1和IRF9,抑制Ljviperin的表达.

结论:

在viperin和VHSV之间存在一种新的反循环,其中病毒逃避viperin的抗病毒作用.
VHSV N 蛋白质通过抑制维培林介导的免疫力,起到毒性因素的作用.
这种相互作用为VHSV预防和抗病毒药物开发提供了潜在的治疗点.

关键词:

自自是一种自的过程.在 IRF1 中,IRF1 是 IRF1 的第一个类型.在 IRF9 中,IRF9 是 IRF9 的第一个类型.免疫逃逸是一种免疫逃避.在Ubiquitination中使用.病毒性出血性败血症病毒病毒

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