通过设计降低免疫原性:降低单克隆抗体免疫原性的方法
Chantal T Harris1, Sivan Cohen2
1Department of BioAnalytical Sciences, Genentech Inc., South San Francisco, CA, 94080-4990, USA.
概括
单克隆抗体 (mAbs) 是重要的治疗药物,但它们的免疫性构成了挑战. 本综述涵盖了在药物开发过程中预测和减少对mAbs的免疫反应的进展.
科学领域:
- 生物技术是生物技术.
- 免疫学 免疫学 免疫学
- 药物开发 药物开发
背景情况:
- 单克隆抗体 (mAbs) 是一种高度特定的蛋白质疗法,用于各种疾病.
- 在mAb疗法中的一个重大挑战是免疫性,一种不必要的免疫反应.
- 免疫消毒策略对于保持 mAb 的有效性和安全性至关重要.
研究的目的:
- 审查最近在预测mAb免疫性方面的进展.
- 突出蛋白质工程战略,以减少免疫性.
- 在mAb开发中强调全面的免疫性风险评估.
主要方法:
- 在体内,体外和体内免疫性风险评估的策略.
- 对驱动 mAb 免疫性机制的分析.
- 对蛋白质工程技术的审查,以消除免疫.
主要成果:
- 免疫原性在 in silico 预测模型中的进展.
- 开发新的蛋白质工程方法,以减少T细胞表皮图识别.
- 改善了对mAb结构与免疫性之间的关系的理解.
结论:
- 综合免疫性评估对于安全有效的mAb疗法至关重要.
- 蛋白质工程提供了强大的工具来减轻mAb免疫性.
- 需要继续进行研究,以完善治疗抗体的预测和缓解策略.
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