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通过一系列分泌的代谢物进行细菌间生物膜竞争

Soniya Rayi¹, Yanyao Cai¹, Jennifer L Greenwich²

¹Department of Chemistry, Indiana University, Bloomington, Indiana 47405, United States.

ACS chemical biology

|January 23, 2024

概括

Pseudomonas aeruginosa 通过化学化合物抑制了Agrobacterium tumefaciens生物膜的形成. 一种pyoverdine前体N5-formyl-N5-hydroxy-l-ornithine (fOHOrn) 被确定为一种新的生物膜抑制剂.

科学领域:

微生物学微生物学
化学生物学化学生物学
生物化学生物化学

背景情况:

多微生物生物膜很常见,但物种间相互作用的化学基础仍然不太清楚.
对抗性相互作用对于构建微生物群落至关重要,并且可能涉及抑制生物膜形成.
众所周知,Pseudomonas aeruginosa产生各种分子,影响细菌的生长和行为.

研究的目的:

阐明 Pseudomonas aeruginosa 抑制 Agrobacterium tumefaciens 生物膜形成的化学机制.
为了识别由P. aeruginosa产生的新型抗菌膜化合物.
研究 siderophores及其前体在物种间生物膜抑制中的作用.

主要方法:

在 siderophore 生产中缺乏 Pseudomonas aeruginosa 突变的构建和分析.
使用化学分析技术识别和描述抗菌膜代谢物.
生物膜抑制试验量化了已识别的化合物对Agrobacterium tumefaciens的影响.

主要成果:

来自P. aeruginosa的三种已知的 siderophores (pyoverdine,pyochelin,dihydroaeruginoic acid) 抑制了A. tumefaciens生物膜的形成.
一种缺乏 siderophores 的 P. aeruginosa 突变仍然产生了抗菌膜代谢产物.
这种代谢物被确定为N5-formyl-N5-hydroxy-l-ornithine (fOHOrn),这是pyoverdine的前体,它抑制了生物膜的形成,没有明显的金属封存.
fOHOrn代表了一种新发现的A. tumefaciens生物膜形成的抑制剂.

结论:

这项研究揭示了N5-formyl-N5-hydroxy-l-ornithine (fOHOrn) 是一种新的,自然产生的抑制剂,可以抑制Agrobacterium tumefaciens生物膜的形成.
这项工作突出了多微生物生物膜中物种间相互作用的复杂性和对抗的化学基础.
这些发现强调了考虑代谢前体的重要性,以了解微生物相互作用和遗传学研究中性质的潜力.

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