底盘和玉米炉生物处理的新陈代谢改造,以从农业废物衍生的基质中解锁3-基酸生物合成
Lei Zhou1, Yufei Zhang1, Tingting Chen1
1School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China.
Journal of agricultural and food chemistry
|January 23, 2024
概括
经过代谢工程的大肠杆菌将玉米炉水解物转化为3-基酸 (3-HP),这是一个有价值的平台化学物质. 这种可持续的生物工艺从农业废物中获得了3-HP的高产量.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 可持续化学 可持续化学
背景情况:
- 农业废物利用对可持续发展至关重要.
- 将纤维素化水解盐转化为3-基酸 (3-HP) 等化学物质是其中的一个关键目标.
研究的目的:
- 设计大肠杆菌以有效地将玉米炉水解盐转化为3-HP.
- 优化新陈代谢途径,以增加3HP的产量.
主要方法:
- 大肠杆菌的新陈代谢重新连接,通过引入糖合成和转换的异质途径.
- 糖醇-3-P脱酶和酸酶 (gpdp12),糖醇脱酶 (DhaB123-gdrAB) 和化脱酶 (GabD4) 的同时表达.
- 5'-非翻译区域工程和代谢调制以增强流量并防止副产品的形成.
主要成果:
- 成功设计了大肠杆菌以从葡萄糖中合成甘油.
- 建立了微调的糖合成和转化途径.
- 防止糖泄漏和中心代谢转移.
- 通过料批发发酵,从纤维素玉米炉水解液中获得32.91g/L的3-HP的最终产量.
结论:
- 大肠杆菌的新陈代谢工程使得3HP从菌纤维素农业废物有效生物生产成为可能.
- 优化途径和代谢控制对于可再生资源的高产化学合成至关重要.
- 这项研究表明,使用农业残留物实现可持续化学制造的可行途径.
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