使用ddRAD测序的印第安巴德里牛的遗传多样性,种群结构分析和代码子替代
Javid Ur Rahman1,2, Devendra Kumar1, Satya Pal Singh3
1Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263145 India.
3 Biotech
|January 23, 2024
概括
对巴德里牛的遗传分析显示,各种颜色变体之间存在很高的相似性,表明缺乏差异化. 该研究确定了特定的遗传密码子替代,为原生牛的保护和品种改进计划提供了见解.
科学领域:
- 动物遗传学动物遗传学
- 人口基因组学 人口基因组学
- 分子进化分子进化
背景情况:
- 巴德里牛表现出鲜明的颜色变体.
- 了解遗传多样性对于保护和品种改进至关重要.
研究的目的:
- 调查巴德里牛的遗传多样性和种群结构.
- 鉴定由单核酸变异引起的基因编码子中的替代突变.
- 评估不同颜色变体之间的遗传相似性.
主要方法:
- 使用双重消化限制相关DNA (ddRAD) 测序对96头巴德里奶牛进行基因定型.
- 生物信息学管道用于单核酸多态 (SNP) 识别和质量过.
- 对遗传多样性指数 (香农,辛普森) 和塔吉马的D.的分析.
- 对人口结构和添加剂的结构分析.
- 对遗传密码变化的SNP的注释.
主要成果:
- 确定了65,483个高度信任的SNP.
- 在黑色,棕色和灰色变种中观察到高遗传相似性 (0.9970-0.9980).
- 结构分析表明添加剂和缺乏遗传差异化.
- 大多数SNP的显著正的Tajima的D值表明了平衡选择.
- 确定了特定的编码子替代,例如,GGC到GGT.
结论:
- 巴德里牛的变种显示出高度的遗传相似性和混合.
- 关于平衡选择和潜在人口收缩的证据.
- 在人群中存在特定的遗传密码子替代.
- 这些发现支持了原住民牛品种的保护和改进战略.
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