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Updated: Jul 5, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

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Cas9和过度活跃的AID之间的合作关系在基准编辑器中建立了广泛和多样化的突变足迹

Kiara N Berríos1, Aleksia Barka1, Jasleen Gill2

  • 1Graduate Group in Biochemistry and Molecular Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Nucleic acids research
|January 23, 2024
PubMed
概括

研究人员通过结合CRISPR-Cas9和DNA除氨酶开发了新的基编辑器 (BEs). 这一突破揭示了这些组件如何合作精确地改变DNA,使得有针对性的基因多样化.

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CRISPR01:59

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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
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Errors During Replication are Corrected by the DNA Polymerase...
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