暴露于极低频电磁场会导致子宫内膜内基因表达的表观遗传调节发生变化
Pawel Jozef Wydorski1, Wiktoria Kozlowska1, Agata Zmijewska1
1Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719, Olsztyn, Poland.
Theriogenology
|January 23, 2024
概括
极低频电磁场 (ELF-EMF) 可以改变猪子宫内膜的表观遗传调节. 这些DNA甲基化和基因表达的变化可能会在怀孕早期影响子宫功能.
科学领域:
- 环境科学 环境科学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 生殖生物学 生殖生物学
背景情况:
- 越来越多的技术发展导致人们广泛接触极低频电磁场 (ELF-EMF).
- 之前的研究表明,ELF-EMF暴露可以影响DNA甲基化水平.
- 表观遗传调节对于子宫功能至关重要,特别是在植入前期.
研究的目的:
- 研究ELF-EMF对猪子宫内膜的表观遗传调节的影响.
- 为了确定ELF-EMF暴露是否会改变子宫内膜中的DNA甲基化,基因素修饰和microRNA生物发生.
- 评估在周植入期间对基因表达和子宫功能潜在的干扰.
主要方法:
- 猪子宫内膜切片在体外暴露在50Hz的ELF-EMF中2小时.
- 关键表观遗传调节因子 (DNMT1,DNMT3a,EZH2,UHRF1,MBD1,DICER1,DGCR8,EED,SUZ12) 的mRNA和蛋白质丰度通过实时PCR和西方涂抹分析.
- 全球DNA甲基化水平和基因素脱乙酶 (HDAC) 活性也被测量.
主要成果:
- 暴露于ELF-EMF显著改变了DNMT1,DNMT3a,EZH2 (mRNA和蛋白质),EED和SUZ12转录的丰度.
- 在ELF-EMF暴露后观察到全球DNA甲基化水平和HDAC活性的变化.
- 参与微RNA生物发生的蛋白质的丰度也受到ELF-EMF治疗的影响.
结论:
- ELF-EMF有可能改变猪子宫内膜中基因表达的表观遗传调节.
- 这些ELF-EMF诱导的表观遗传变化可能导致子宫内膜转录基因特征的变化.
- 这种干扰可能会破坏子宫在植入前阶段的关键生物过程.
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