新出现的超冰川湖中繁殖的细菌拥有金属和抗生素耐药基因
Wasim Sajjad1, Nikhat Ilahi2, Abdul Haq3
1State Key Laboratory of Cryospheric Science, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.
Environmental research
|January 23, 2024
概括
抗生素耐药性基因 (ARG) 和耐金属基因 (MTG) 在偏远的上冰川湖细菌中被发现. 这些基因同时发生和转移,突出显示原始生态系统中潜在的环境风险.
科学领域:
- 环境微生物学环境微生物学
- 分子生物学分子生物学
- 冰川学的冰川学
背景情况:
- 抗生素耐药性 (AR) 是一个重要的全球健康问题,在临床和自然环境中广泛研究.
- 抗生素耐药基因 (ARG) 在原始的,偏远的生态系统 (如上冰川湖) 的存在和传播在很大程度上尚未被探索.
- 超冰川湖是新形成的环境,可能有独特的微生物群落,这对基因转移有影响.
研究的目的:
- 调查ARG和金属耐受性基因 (MTGs) 在来自偏远的上冰川湖环境的细菌菌株中的患病率.
- 评估ARG和MTG在这些孤立的生态系统中的共同发生和潜在的共同选择.
- 探索这些基因在细菌分离物中水平基因转移 (HGT) 的证据.
主要方法:
- 细菌菌株从巴基斯坦杜克帕尔冰川 (Dook Pal Glacier) 上一个超冰川湖的碎片和融水中分离出来.
- 传统的聚合酶链反应 (PCR) 用于检测特定的ARG (例如,blaTEM,qnrA) 和MTG (例如,copA, czcA).
- 使用统计分析来确定ARG和MTG存在之间的相关性.
主要成果:
- 在来自湖泊碎片 (92.5%) 和融水 (100%) 的细菌分离物中观察到ARG的高患病率.
- 经常检测到像blaTEM和qnrA这样的特定ARG,qnrA在碎片中占主导地位,两者都在融水中占主导地位.
- 绝缘物中很大一部分还含有MTG (92.5%在碎片中,83.3%在融水中),其中 czcA 在碎片中占主导地位,而 copA 在融水中占主导地位.
- 显而易见的是,ARG和MTG的同时发生,以及证据表明不同细菌物种之间的水平基因转移 (HGT).
- 在ARG和MTG存在之间发现了积极和消极的相关性.
结论:
- 遥远的超冰川湖环境在其细菌群体中藏着各种各样的抗生素耐药性基因和金属耐受性基因.
- ARG和MTG的同时出现和潜在的共同选择,以及HGT的证据,表明这些原始生态系统中的复杂微生物动态.
- 这些发现强调了研究偏远环境的重要性,以了解抗菌素耐药性的全球传播以及进一步研究的必要性.
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