来自Inonotus hispidus的异型多糖的结构特征,链形状和免疫调节活性
Xiaopeng Liu1, Qiuyi Wang1, Jie Wang1
1National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, Henan, China; College of Agriculture, Henan University, Kaifeng 475004, China.
International journal of biological macromolecules
|January 23, 2024
概括
一种新型的多糖体,IHP-1aa,从Inonotus hispidus中分离出来. 这种独特的异质多糖体显示出作为免疫调节剂的潜力,增强巨细胞功能和细胞因子分泌,用于功能性食品应用.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
背景情况:
- Inonotus hispidus是一种药用,含有各种生物活性化合物.
- 来自真菌的多糖因其免疫调节特性而闻名.
- 对新型真菌多糖的表征对于发现新的治疗剂至关重要.
研究的目的:
- 从Inonotus hispidus的果实体中分离和表征一种新的多糖 (IHP-1aa).
- 研究IHP-1aa.aa的潜在免疫调节作用.
- 探索IHP-1aa在功能性食品中的应用.
主要方法:
- 热水提取,乙醇沉和柱状染色学被用于多糖分离.
- 结构分析涉及诸如SEM,XRD,AFM和刚果红色试验等技术.
- 使用RAW264.7巨细胞进行了体外试验,以评估免疫调节活性.
主要成果:
- 一种新的异多多糖,IHP-1aa (26.9 kDa),已经成功分离出来.
- IHP-1aa由葡萄糖,银河糖,糖,曼诺糖和3-O-甲基银河糖组成,具有特定的主链和侧链结构.
- IHP-1aa显著刺激了巨细胞的功能,增强了细胞分裂,并促进了氧化 (NO),瘤坏死因子-α (TNF-α) 和介素-6 (IL-6) 的分泌.
结论:
- IHP-1aa是一种独特的含有3-O-甲基银糖的异多聚糖,从I. hispidus中分离出来.
- 这项研究提供了IHP-1aa.的免疫调节潜力的证据.
- 在功能性食品中,IHP-1aa可以作为一种有价值的成分来支持免疫系统.
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