全基因组关联研究确定了葡萄牙美利诺羊足迹的基因变异
Daniel Gaspar1,2,3, Catarina Ginja2,3,4, Nuno Carolino4,5,6
1Centro de Biotecnologia Agrícola E Agro-Alimentar Do Alentejo (CEBAL)/ Instituto Politécnico de Beja (IPBeja), 7801-908, Beja, Portugal.
BMC genomics
|January 23, 2024
概括
研究人员确定了与绵羊脚抗性相关的遗传标记. 这项关于葡萄牙美利诺羊的研究为改善疾病控制和动物福利的育种计划提供了见解.
科学领域:
- 动物遗传学动物遗传学
- 兽医科学 兽医科学 兽医科学
- 绵羊生产 绵羊生产
背景情况:
- 由Dichelobacter nodosus引起的绵羊脚,在全球范围内显著影响了绵羊生产.
- 了解宿主遗传耐药性对于有效的疾病控制策略至关重要.
- 这项研究重点关注葡萄牙本土的美利诺品种和杂交种群.
研究的目的:
- 为了确定与羊的footrot耐药性和易感性相关的遗传变异.
- 为了探索葡萄牙美利诺羊的足迹的遗传结构.
- 为增强绵羊育种计划提供分子标记物.
主要方法:
- 全基因组关联研究 (GWAS) 使用定制的SNP数组 (47,779个SNP).
- 在葡萄牙阿伦泰乔地区对17个羊群中的1375只绵羊进行了分析.
- 使用修改的埃格顿系统进行表型评分 (0=健康,5=严重的脚).
- 线性混合模型方法与邦费罗尼对统计显著性的纠正.
主要成果:
- 24号染色体上的三个单核酸多态 (SNP) 达到全基因组显著性 (p < 0.05).
- 在染色体2,4,7,8,9和15上发现了另外6个暗示性SNP.
- 已发现的候选基因包括SMG1 (免疫),RALYL (免疫),HSPG2 (组织修复) 和THBS1 (伤口愈合).
结论:
- 这是第一个在葡萄牙美利诺羊中识别footrot分子标记的研究.
- 这些发现突出了潜在的遗传关联和候选基因,这些基因会影响足抗性/易感性.
- 结果可以为在绵羊养殖中可持续的,长期的脚控制的遗传选择策略提供信息.
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