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Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
Published on: April 9, 2012
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为Xpert MTB/RIF测定汇集唾液样本:对于高度传染性结核病例的实用查策略
Jianfeng Zeng1, Huan Huang1,2, Xuhui Liu1
1Shenzhen Third People's Hospital, National Clinical Research Center for Infectious Disease, 518112, Shenzhen, China.
BMC infectious diseases
|January 23, 2024
概括
使用Xpert MTB/RIF测试 (Xpert) 进行结核病 (TB) 查的唾液样本组合保持了高灵敏度,特别是在传染病患者中. 这种具有成本效益的策略可以在资源有限的环境中改善结核病检测.
科学领域:
- 微生物学 微生物学
- 公共卫生 公共卫生
- 医学诊断 医学诊断 医学诊断
背景情况:
- 专家MTB/RIF测试 (Xpert) 对于结核病 (TB) 诊断至关重要,但由于成本,它面临着可访问性挑战.
- 唾液样本组合是降低成本和扩大结核病查覆盖范围的潜在策略.
- 混合样本的稀释效应可能会降低Mycobacterium结核病 (Mtb) 检测灵敏度.
研究的目的:
- 用Xpert测定来评估不同混合比对聚合唾液样本检测性能的影响.
- 确定最佳的聚合策略,以最大限度地提高结核病查的敏感性和成本效益.
主要方法:
- 使用了来自确诊结核病患者和非结核病患者的冷唾液样本.
- 样品以各种比例 (例如1/2,1/8,1/16) 聚合,并使用Xpert MTB/RIF测定进行测试.
- 检测率是根据混合比率和患者特征 (如Mtb涂抹得分和培养结果) 分析的.
主要成果:
- 在混合比为1/2至1/8的情况下,总体灵敏度保持在80%以上.
- 在1/16的混合比率下,灵敏度降至69.0%.
- 对于具有高Mtb负载指标的患者,高灵敏度 (≥93%) 保持在1/16的比例 (涂抹得分≥2+,快速培养或高/中等细菌丰度).
- 在患有腔 (86.2%) 和咳 (90.0%) 的患者中,聚合试验的阳性率更高,比例为1/8.
结论:
- 专家聚合试验显示出高灵敏度,特别是在具有高Mtb传染性的人群中.
- 这种节约成本的聚合策略可以在资源有限的地区加强结核病查.
- 实施聚合测试可以有助于减少全球结核病传播和发病率.
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