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Updated: Jul 5, 2025

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

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C-to-G编辑会产生双链断裂,导致删除,转换和转位.

Min Emma Huang1,2, Yining Qin2, Yafang Shang1,2

  • 1Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China.

Nature cell biology
|January 23, 2024
PubMed
概括

基编辑器 (BEs) 可以导致DNA双链断裂 (DSB),导致不必要的副产品. 用HMCES保护基底部部位可以减少这些DSB,使基底编辑更安全,用于治疗.

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