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DNA甲基化和染色质可访问性可以预测家养狗的年龄

Kelly Jin¹, Brianah M McCoy^2,3, Elisabeth A Goldman⁴

¹Department of Laboratory Medicine & Pathology, University of Washington, Seattle, Washington, USA.

|January 24, 2024

概括

表观遗传时钟使用DNA甲基化 (DNAm) 或染色质可访问性来预测年龄. 这项研究表明,染色质可访问性是狗生物年龄的可行,互补的生物标志物,识别与神经功能相关的基因.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
基因组学就是基因组学.
比较生物学的比较生物学

背景情况:

主要基于DNA甲基化 (DNAm) 的表观遗传时钟是哺乳动物衰老的已知生物标志物.
其他表观遗传特征的预测能力,如染色质可访问性,对时间年龄的预测能力仍然不太了解.

研究的目的:

研究染色质可访问性的实用性,与DNA甲基化一起,用于预测狗的时间年龄.
开发和比较使用DNAm,ATAC-seq和狗外周血液单核细胞的综合数据的表观遗传钟.

主要方法:

从71只狗身上生成了DNA甲基化 (DNAm) 和ATAC-seq资料.
使用这些数据集构建了单独和组合的表观遗传钟.
光辅助的细胞分类量化了主要的淋巴细胞群.

主要成果:

染色体可访问性 (ATAC-seq) 预测年龄与26%的差异解释 (R2),DNAm预测年龄与33%的差异解释 (R2),并结合数据预测年龄与29%的差异解释 (R2).
特定的CD62L+T细胞种群与时间年龄有显著的相关性.
选择的时钟特征位于BAIAP2和SCARF2基因附近,这些基因与神经过程有关.

结论:

染色体可访问性作为一种有价值的,补充表观遗传生物标志物,用于对狗的生物年龄进行评估.
这项研究扩大了表观遗传钟的应用范围,超越了DNA甲基化,为衰老研究提供了新的途径.

关键词:

在ATAC-seqq中使用.通过DNA甲基化.老化的老化衰老的老化这些狗狗狗狗狗狗狗狗.表观遗传时钟的时间表.

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