瘤和肌层之间的交叉声调整了细胞外囊中的CLIC4载荷
Vanesa C Sanchez1,2, Alayna Craig-Lucas3,2, Christophe Cataisson2
1Current address: Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, 20993.
Journal of extracellular biology
|January 24, 2024
概括
氧化还原酶CLIC4 (化物细胞内通道4) 的宿主表达对于乳腺癌肺转移的发展至关重要. 瘤细胞释放含有CLIC4的EVs,但 stromal因素影响其结合,影响转移性播种.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 癌症转移 癌症转移
背景情况:
- 乳腺癌转移到肺部是一个重大的临床挑战.
- 肺部形成一个前转移性位对于成功的转移性播种至关重要.
- 特定宿主因子在促进转移中的作用尚未完全理解.
研究的目的:
- 为了研究氧化还原酶CLIC4在乳腺癌肺转移中的作用.
- 确定在循环中的细胞外囊泡 (EVs) 中CLIC4的来源和调节.
- 探索宿主流体对电动汽车货物整合的影响.
主要方法:
- 利用乳腺癌的小鼠模型,在宿主和瘤细胞中改变CLIC4表达.
- 对CLIC4含量进行了分析.
- 评估了肺前转移性和转移性播种的发展.
- 在野生类型和CLIC4淘汰主机环境中比较EV货物.
主要成果:
- 主体CLIC4表达对于肺转移是必需的;瘤生长,但没有它,前转移性位是有缺陷的.
- 初级乳腺癌细胞释放含有CLIC4的EV,这些EV存在于瘤载体宿主的血中.
- 缺乏CLIC4的瘤细胞会释放缺乏CLIC4的EVs.
- 当CLIC4表达瘤在CLIC4淘汰主体中生长时,循环EV中没有CLIC4,这表明 stromal的影响.
结论:
- 主体CLIC4对于建立乳腺癌转移的肺前转移性利基至关重要.
- 来自瘤的EVs携带CLIC4,但其结合是由宿主 stromal 信号调节的.
- 循环EV中的CLIC4可能作为乳腺癌进展的潜在生物标志物.
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