纳米颗粒的氨酸表面密度控制非内细胞细胞吸收和自诱导
Reeddhi Ray1, Santu Ghosh1, Anupam Maity1
1School of Materials Science, Indian Association for the Cultivation of Science, Kolkata 700032, India.
ACS applied materials & interfaces
|January 24, 2024
概括
纳米颗粒表面的氨酸密度和距离是非内细胞细胞吸收的关键. 距离低于3nm便于直接穿透膜,增强细胞活动和生物医学应用.
科学领域:
- 纳米技术 纳米技术
- 细胞生物学 细胞生物学
- 生物医学工程 生物医学工程
背景情况:
- 纳米材料的非内细胞吸收很困难,通常需要小颗粒大小和特定的表面化学.
- 氨酸终结纳米颗粒 (<10-20nm) 已经显示通过直接穿透膜的非内细胞吸收.
- 氨酸密度和氨酸间距离对这种吸收机制的影响仍然未被探索.
研究的目的:
- 研究表面氨酸密度和氨酸-氨酸距离在控制非内细胞纳米粒子吸收中的关键作用.
- 为了阐明纳米颗粒通过非内细胞通路进入细胞的细胞内作用.
- 为提高纳米载体和纳米探针性能建立设计原则.
主要方法:
- 使用基于量子点 (QD) 的纳米粒子作为光可追溯模型.
- 合成的氨酸终结纳米颗粒具有受控大小 (10纳米) 和不同的氨酸-氨酸距离.
- 通过光显微镜和生物化学试验量化评估细胞吸收和细胞内定位.
主要成果:
- 较高的氨酸密度和氨酸-氨酸距离<3nm极大地促进了非内细胞的吸收.
- 具有氨酸-氨酸距离>3nm的纳米颗粒通过能量依赖性内细胞分解被内部化.
- 氨酸-氨酸距离<3nm导致了快速的非内细胞进入,细胞质积累,ATP耗尽,氧化应激和自诱导.
结论:
- 氨酸 - 氨酸距离是基本参数,与粒子大小一起,控制非内细胞纳米材料的吸收.
- 控制氨酸间距离 (<3 nm) 能够有效地进入非内细胞并操纵细胞内过程.
- 这一策略可以优化纳米探测器和纳米载体的设计,以改善生物医学应用.
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