用替代的合聚电解质显示出有效的可见光诱导的抗菌活性
Han Sun1, Isaí Barboza-Ramos1, Xiaodan Wang1
1Department of Chemistry, University of Texas, San Antonio, 1 UTSA Circle, San Antonio, Texas 78249, United States.
ACS applied materials & interfaces
|January 24, 2024
概括
新的替代联多电解质显示出强大的光激活抗菌活性. 这些材料有效地杀死细菌,如大肠杆菌和黄金色杆菌通过反应性氧物种在照明时生成.
科学领域:
- 材料科学 材料科学 材料科学
- 摄影化学的使用.
- 微生物学 微生物学
背景情况:
- 合聚电解质 (CPE) 是具有独特电子和光学特性的聚合物.
- 含的化合物可以表现出抗菌性质.
- 导致反应性氧物种 (ROS) 的光敏感化是一种已知的抗菌机制.
研究的目的:
- 合成和表征用替代的合聚电解质 (CPE).
- 评估这些CPE在体外抗菌活性对抗氏阴性和氏阳性细菌.
- 调查背后的机制,无论是光激活和黑暗的抗菌活性.
主要方法:
- 合成基于聚乙乙烯 (PPE) 的CPE,其酸侧链长度不同.
- 在体外使用大肠杆菌和金黄色葡萄球菌的抗菌试验.
- 暴露在黑暗和照明条件 (蓝色LED) 中,CPE度各不相同.
- 减少殖民地形成单位 (CFU) 和连续稀释涂层以量化细菌不活化.
主要成果:
- 在低度 (≤20μM) 的照明下,CPE表现出显著的光激活抗菌活性,在照明下实现99%以上的CFU降低对大肠杆菌和金黄色细菌.
- 观察到暗活动,特别是针对金黄色,与CPE疏水性和侧链长度相关.
- 光激活活动与ROS生成有关,而暗活动与细菌膜破坏有关.
- 在暴露于蓝光后15分钟内,E. coli的杀死率超过5日志.
结论:
- 用替代的CPE是有效的光激活抗菌剂.
- 通过修改CPE侧链长度,可以调整抗菌功效.
- 这些材料为光动力学抗菌疗法提供了一个有前途的平台.
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