O-GlcNAcylation通过维持线粒体动态和功能来协调猪卵细胞的成熟
Wen-Jie Xiong1, Xin-Le Lai1, Jie Lu2
1Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China.
Molecular human reproduction
|January 24, 2024
概括
与O相关的β-N-乙糖胺 (O-GlcNAc) 对细胞过程至关重要. 在猪卵细胞中抑制O-GlcNAc转移酶 (OGT) 会破坏成熟,影响细胞结构,线粒体,并导致氧化应激,突出O-GlcNAc.
科学领域:
- 细胞生物学 细胞生物学
- 生殖生物学 生殖生物学
- 生物化学 生物化学
背景情况:
- 与O结合的β-N-乙糖胺 (O-GlcNAc) 是一种动态的翻译后修饰,调节转录和新陈代谢等关键细胞过程.
- O-GlcNAc的修饰是由O-GlcNAc转移酶 (OGT) 和O-GlcNAcase控制的.
- 对于O-GlcNAc修饰在女性生殖健康,特别是卵细胞质量方面的具体作用尚不清楚.
研究的目的:
- 调查O-GlcNAc修饰在猪卵细胞成熟和质量的功能作用.
- 阐明OGT抑制对卵细胞内细胞事件的影响.
主要方法:
- 在猪卵细胞中抑制O-GlcNAc转移酶 (OGT).
- 卵细胞成熟的评估,包括第一个极体挤出.
- 对actin和微管组件的分析.
- 评估线粒体动力学和功能.
- 测量氧化应激和自标志物.
主要成果:
- OGT抑制阻止了第一个极体挤出,表明卵细胞成熟失败.
- 观察到actin和微管细胞骨架组织的异常.
- 线粒体动力学和功能显著受到干扰.
- 抑制导致氧化应激增加和诱导自.
结论:
- O-GlcNAc的糖化对成功的猪卵细胞成熟至关重要.
- 破坏O-GlcNAc修饰会影响细胞骨完整性,线粒体健康和细胞应激反应.
- 对O-GlcNAc在卵细胞细胞事件中的调节作用的进一步研究是有必要的.
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