乳腺癌中转移细胞的特异表达从固体瘤中分离出来的介质干细胞
Zahra Sadat Hashemi1,2,3, Mehdi Forouzandeh Moghadam4, Saeed Khalili5
1Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Current stem cell research & therapy
|January 24, 2024
概括
介酶干细胞 (MSCs) 影响瘤生长和转移. 这项研究揭示了乳腺癌MSCs (BC-MSCs) 中的特定microRNA (miR) 表达,这表明miR疗法是乳腺癌入侵的潜在治疗方法.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 介酶干细胞 (MSC) 是瘤微环境的组成部分,分泌影响瘤生长和转移的因素.
- 转基因Rs是新兴的转基因调节器,特别涉及上皮细胞到介质细胞转换 (EMT).
研究的目的:
- 为了研究乳腺癌相关的MSCs (BC-MSCs) 中转移性Rs的表达特征.
- 为了将BC-MSC中的转移性R表达与它们的侵入性和迁移性能力相关联.
- 探索针对乳腺癌中这些miRs的潜在治疗策略.
主要方法:
- 瘤分离的BC-MSCs和正常的人类乳腺上皮细胞 (HMECs) 的表征,以及乳腺癌细胞系 (MCF-7,MDA-MB231,MCF-10A).
- 使用生物信息学数据库 (GEO,KEGG,TCGA) 评估细胞表面标记物 (CD44,CD24),茎状标记物 (Oct-4,Survivin) 和微分RNA (miR) 表达.
- 对13 miR和Transwell-Matrigel测定进行定量实时PCR,以评估细胞迁移和入侵.
主要成果:
- 在BC-MSC中观察到的oncomiR miR-10b的升调;miR-373和miR-520c水平与MCF-10A相当.
- 与miR-146a,miR-146b和miR-335相比,miR-200家族成员在BC-MSC中表达较低.
- 在MCF-10A细胞中,miR-31和miR-193b被上调,而MDA-MB231表现出最高的侵入性.
结论:
- BC-MSC miR表达特征位于MCF-7和MDA-MB231之间,这可能解释了它们的中等入侵水平.
- 这些发现支持miR治疗的潜力,使用miR模仿剂或antagomiRs等药物,用于通过BC-MSCs治疗乳腺癌的临床应用.
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