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植物U盒E3连接酶PUB20和PUB21负调节Arabidopsis中模式触发的免疫力

So Young Yi^1,2, Vladimir Nekrasov^3,4, Kazuya Ichimura⁵

¹Center for Sustainable Resource Science, RIKEN, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan. yisy@kongju.ac.kr.

Plant molecular biology

|January 24, 2024

概括

植物U盒E3连接酶PUB20和PUB21对植物免疫进行负面调节. 这些蛋白质的损失通过促进早期防御反应和口腔免疫来增强对细菌入侵的抵抗力.

科学领域:

植物免疫力植物免疫力
分子植物病原体相互作用
E3 无处不在酸接酶

背景情况:

植物U-box (PUB) E3酶参与压力反应.
PUB20和PUB21是密切相关的IV类PUB,具有C端阿马迪略重复.
了解它们在植物免疫中的作用对于作物保护至关重要.

研究的目的:

研究PUB20和PUB21在植物天生的免疫力中的功能.
确定PUB20和PUB21在对细菌病原体的反应中的作用.
阐明PUB20和PUB21规范的信号通路.

主要方法:

产生PUB20和PUB21 (pub20-1,pub21-1,pub20-1/pub21-1) 的淘汰突变物.
评估对Pseudomonas syringae pv.的耐药性进行评估番茄DC3000 (Pst DC3000) 通过表面和透接种.
测量flg22诱导的活性氧生产,MPK3激活和免疫基因表达.
分析口腔关闭反应.

主要成果:

PUB20和PUB21可以负面调节植物对Pst DC3000的免疫力.
失去PUB20/PUB21功能会增强抵抗力,尤其是在早期感染阶段.
PUB突变体表现出增加的flg22触发反应性氧气生产,MPK3激活和免疫基因表达.
PUB突变体表现出构成性口腔关闭,表明PUB20在口腔免疫中的作用.
由RPM1/avrRpm1和RPS2/avrRpt2介导的过敏反应与PUB20/PUB21无关.

结论:

PUB20和PUB21作为植物天生的免疫力的负调节剂.
这些E3链酶在flg22触发的信号通路中起作用.
PUB20和PUB21在早期防御阶段影响细菌入侵和口腔免疫.

关键词:

阿拉比多普西斯 (Arabidopsis) 是一种植物.E3 结合酶的使用在PUB20中,PUB20是PUB20的版本.在PUB21中,PUB21是由模式触发的免疫力

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