相关实验视频
Updated: Jul 5, 2025
07:02
Polysome Purification from Soybean Symbiotic Nodules
Published on: July 1, 2022
1.5K
在豆类共生细菌中非编码RNA的功能性特征化工作流程
Natalia I García-Tomsig1, Sabina K Guedes-García1, José I Jiménez-Zurdo2
1Structure, Dynamics and Function of Rhizobacterial Genomes (RhizoRNA Lab), Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Granada, Spain.
Methods in molecular biology (Clifton, N.J.)
|January 24, 2024
概括
本研究详细介绍了在Sinorhizobium meliloti.中分析细菌小RNA (sRNA) 的方法. 这些技术有助于揭示sRNAs在非模型细菌的基因表达中的调节作用.
科学领域:
- 细菌分子生物学 细菌分子生物学
- 基因组学就是基因组学.
- RNA生物学的RNA生物学
背景情况:
- 小型非编码RNAs (sRNAs) 调节细菌中的基因表达.
- 它们在大多数非模型细菌中的作用尚未得到充分研究.
- 这些调节性RNA通过反意义相互作用微调基因表达.
研究的目的:
- 编制用于研究Sinorhizobium meliloti.中跨作用sRNA (跨sRNA) 的实验方法.
- 为研究α-rhizobia中的sRNA功能提供工具.
- 为了使细菌sRNAs的检测,表征和目标验证.
主要方法:
- 用于转录组分析的RNA测序 (RNAseq).
- 检测sRNA表达和确定5'/3'-end. 的方法.
- 评估转录调节和产生突变的技术.
- 验证跨-sRNA的目标mRNAs.
主要成果:
- 介绍了分析细菌转基因sRNA的全面工作流程.
- 这些方法适用于Sinorhizobium meliloti和相关细菌.
- 这项研究有助于对新发现的sRNAs进行功能性表征.
结论:
- 开发的方法支持在非模型细菌中调查sRNA调节网络.
- 这项工作增强了对α-rhizobia中转录后基因调节的理解.
- 它为探索不同细菌物种的sRNA功能提供了基础.
相关概念视频
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K