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达利对DPP的扩散或内部化并不重要,而是通过对抗Tkv介导的DPP内部化,对DPP的稳定性至关重要

Niklas Simon¹, Abu Safyan^2,3,4,5,6, George Pyrowolakis^3,4,5,6

¹Growth & Development, Biozentrum, Spitalstrasse, University of Basel, Basel, Switzerland.

|January 24, 2024

概括

异常延迟的分裂 (Dally) 对Drosophila翅膀盘的发育至关重要. 它的核心蛋白与DPP相互作用,而它的肝硫酸盐链通过防止细胞内化来稳定DPP.

科学领域:

发展生物学发展生物学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

肝酸硫酸蛋白质甘 (HSPGs) 是关键的细胞表面分子,调节形态原梯度的形成和信号传递.
在Drosophila翅膀盘中,Glypicans异常延迟的 (Dally) 和类似Dally的 (Dlp) 被认为可以通过它们的肝酸盐 (HS) 链冗余地控制Dpp形态变态梯度.
之前的模型建议glypicans促进或阻碍DPP传播,稳定DPP或回收DPP,但不同的HSPG功能仍然不清楚.

研究的目的:

阐明Dally和DLP在控制Dpp形态变态梯度形成和Drosophila翅膀盘中的信号传递中的不同作用.
为了研究达利的核心蛋白和HS链对DPP梯度调节的特定贡献.

主要方法:

为Drosophila中的Dally和Dlp生成基因组工程平台.
在DPP梯度形成和信号中分析Dally和DLP函数.
对Dally-Dpp和Dally HS-Dpp相互作用的研究.

主要成果:

只有Dally,而不是Dlp,被发现对Dpp梯度形成和信号发送至关重要.
达利的核心蛋白与DPP相互作用,但这种相互作用本身不足以发挥作用.
达利的HS链对于DPP梯度形成和信号传递至关重要,主要是通过稳定DPP在细胞表面.
达利的HS链对抗受体介导的DPP内部化,而不是促进DPP传播或回收.

结论:

达利在Drosophila翅膀盘中的Dpp形态基因梯度控制中发挥着关键的,非冗余的作用.
达利通过涉及其核心蛋白质和HS链的双重机制调节DPP信号.
达利的HS链通过抑制其内部化来稳定Dpp,为形态原梯度调节提供了一种新的机制.

关键词:

D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. melanogaster. D. melanogaster. melanogaster. D. melanogaster. D. melanogaster. melanogaster. D.达利·达利 (Dally Dally) 是一个名为达利·达利 (Dally Dally) 的名人.在DPP/BMP中使用.细胞生物学细胞生物学发育生物学是发展生物学.格莱皮卡纳 (Glypican) 是一种葡萄糖.形态原体是一种形态原体.机翼盘机翼盘机翼盘机翼盘

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