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通过机器学习介导的Passiflora caerulea调生成优化优化
Marziyeh Jafari1,2, Mohammad Hosein Daneshvar2
1Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran.
PloS one
|January 24, 2024
概括
机器学习可以准确地预测Passiflora caerulea callogenesis的发生. 使用遗传算法优化植物生长调节器 (PGR) 和扩展型最大限度地提高了产量,为组织培养进步提供了强大的工具.
科学领域:
- 植物生物技术 植物生物技术
- 计算生物学 计算生物学
- 农业科学 农业科学
背景情况:
- 呼生成对于Passiflora caerulea在体外二次代谢物生产和间接器官生成至关重要.
- 优化呼叫生成协议需要先进的计算方法.
研究的目的:
- 使用机器学习 (ML) 预测Passiflora caerulea中的生反应.
- 为了优化植物生长调节剂 (PGR) 度和扩增类型,以增强生.
- 通过灵敏度分析,评估PGR和扩展型对产生的影响.
主要方法:
- 多层感知子 (MLP) 用于建模生率和的新鲜重量.
- 爆炸物类型 (叶子,节点,内部节点) 和PGRs (2,4-D,BAP,NAA,IBA) 是不同的.
- 将MLP模型与遗传算法 (GA) 集成,用于优化和灵敏度分析.
主要成果:
- 对于呼生成参数,MLP表现出高预测精度 (R2>0.81).
- 确定了100%的生率的最佳条件:叶子扩张,具有特定度的IBA,NAA,2,4-D和BAP.
- 敏感性分析揭示了PGRs对产生的解释性依赖作用.
结论:
- MLP和GA的组合有效地优化和预测Passiflora的体外培养系统.
- 这种方法提供了一个前性的解决方案,以应对Passiflora组织培养的挑战.
- 这些发现促进了高效的二次代谢物生产和间接的器官生成.
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