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Updated: Jul 5, 2025

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
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单分子FRET在10MHz的计数速率

Lennart Grabenhorst1, Flurin Sturzenegger2, Moa Hasler2

  • 1Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, 81377 München, Germany.

Journal of the American Chemical Society
|January 24, 2024
PubMed
概括

研究人员开发了DNA纳米天线,以提高单分子Förster共振能量转移 (smFRET) 研究的光子计数率. 这一突破使得以前无法观察到的超快速生物分子动力学成为可能.

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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