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优化混合凝-多糖化物生物墨水利用醇-诺伯化学利用减少添加剂的优化

Nathan Carpentier¹, Laurens Parmentier¹, Louis Van der Meeren²

¹Polymer Chemistry & Biomaterials Group, Centre of Macromolecular Chemistry, Department of Organic and Macromolecular Chemistry, Ghent University, 9000 Ghent, Belgium.

Biomedical materials (Bristol, England)

|January 24, 2024

概括

这项研究探讨了使用tris(2-carboxyethyl) (TCEP) 来改善醇-诺伯烯水凝生物打印. 最佳的TCEP度可以防止二硫化物形成和自化,确保稳定的生物油墨粘度和生物相容性.

科学领域:

生物材料科学生物材料科学
聚合物化学聚合物化学
生物技术是生物技术.

背景情况:

醇 - 诺伯烯化学物质使步骤增长的水凝交叉连接成为可能.
硫化聚合物中的氧气诱导的二硫化物形成增加了粘度,阻碍了生物打印.
二硫化物形成是含醇的水凝生物打印的关键限制.

研究的目的:

调查使用tris(2-carboxyethyl) (TCEP) 缓解二硫化物形成在生物打印的norbornene-modified dextran (DexNB) 和硫化凝 (GelSH) 水凝.
为了确定一个最佳的TCEP度,防止过早的交叉连接,同时保持生物油墨的特性.
评估TCEP对水凝网络同质性和细胞活性的影响.

主要方法:

准备的诺伯改性德克斯 (DexNB) 和硫化凝 (GelSH).
加入不同度的三2-碳氧乙基啡 (TCEP).
H-NMR光谱检测用于监测硫酸盐和硫酸盐的反应和二硫化物形成.
风湿学测量以评估粘度稳定性.
原子力显微镜 (AFM) 用于网络同质性分析.
细胞活力测定 (ISO 10993-6:2016) 使用人类前皮纤维细胞.

主要成果:

在DexNB/GelSH水凝中,TCEP有效地防止了二硫化物形成.
确定了最佳的TCEP度 (25mol%相对于硫酸盐),最大限度地减少了二硫化物形成和自发的硫酸盐-非硫酸盐交叉连接.
优化的水凝配方表现出稳定的粘度超过24小时.
随着TCEP的加入,AFM揭示了一个更为均的网络结构.
人前皮纤维细胞的细胞活力超过了70%,表明具有良好的生物相容性.

结论:

TCEP是一种有价值的添加剂,可以通过防止过早的二硫化物交叉连接来提高醇-诺波烯水凝的生物打印能力.
一个优化的TCEP度平衡了防止二硫化物形成,最大限度地减少不必要的自化.
开发的水凝系统显示了先进生物打印应用的潜力,这些应用需要稳定和生物相容的生物墨水.

关键词:

生物墨水是生物墨水.生物打印是一种生物打印技术.无论是原生改性生物聚合物还是原生改性生物聚合物化生物聚合物化生物聚合物粘度粘度粘度粘度粘度

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