相关实验视频

Updated: Jul 5, 2025

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

珀色LED对炎症和调节性单细胞和淋巴细胞的影响

Kaline de Brito Sousa¹, Tainá Caroline Dos Santos Malavazzi¹, Maria Fernanda Setúbal Destro Rodrigues¹

¹Postgraduate Program in Medicine-Biophotonics, Nove de Julho University (UNINOVE), 235/249 Vergueiro Street, Liberdade, São Paulo, SP 01504-001, Brazil.

Journal of photochemistry and photobiology. B, Biology

|January 24, 2024

概括

珀LED光生物调节 (PBM) 影响人类免疫细胞. 这项研究发现,PBM改变单细胞和淋巴细胞中的光吸收和细胞因子表达,影响炎症和修复过程.

科学领域:

免疫学免疫学免疫学
生物光子学生物光子学
细胞生物学细胞生物学

背景情况:

光生物调制 (PBM) 使用光来影响细胞功能.
珀LED光 (590nm) 正在探索其治疗潜力.
单细胞和淋巴细胞在免疫反应和组织修复中起着至关重要的作用.

研究的目的:

研究珀色LED PBM对人类单细胞和淋巴细胞的影响.
评估PBM对细胞的影响,分化为促炎性和调节性表型.
分析细胞吸收谱和细胞因子表达的变化.

主要方法:

使用LPS或LPS+IL-4进行人体白细胞两极分化.
细胞被珀色LED (590nm) 照射在2小时和6小时.
细胞吸收,基因和蛋白质表达 (IL-1β,IL-6,IL-10,IL-17,TNF-α,IFNγ) 在24小时后被测量.

主要成果:

珀LEDPBM改变了极化单细胞和非极化淋巴细胞的吸收光谱.
照射降低了亲炎性单细胞中的IL-6和TNF-α.
在调节性单细胞和淋巴细胞中,PBM降低了IL-10,同时在淋巴细胞中增加了IL-17.

结论:

珀LED PBM调节单细胞和淋巴细胞中的光吸收和细胞因子表达.
这些效应表明PBM会影响炎症和修复过程.
进一步的研究可以探索PBM在免疫调节中的治疗应用.

关键词:

这是一个珀珀.在LEDLEDLEDLEDLEDLED上,我们可以看到白血球是白血球的细胞.光学吸收率是指光学吸收率.摄影生物调制 (Photobiomodulation) 是一种生物调制方法.

更多相关视频

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Published on: August 12, 2019

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

相关概念视频

Inflammatory Response

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...