VO2微/纳米颗粒对固定的细菌阿佐托巴克特维尼兰迪的毒性
Peng Ouyang1, Jinwei Yang2, Qinmei Zhong2
1Key Laboratory of Pollution Control Chemistry and Environmental Functional Materials for Qinghai-Tibet Plateau of the National Ethnic Affairs Commission, Key Laboratory of General Chemistry of the National Ethnic Affairs Commission, School of Chemistry and Environment, Southwest Minzu University, Chengdu 610041, China; College of Resources and Environment, Southwest University, Chongqing 400715, China.
Journal of hazardous materials
|January 24, 2024
概括
二氧化物 (VO2) 伤害固菌Azotobacter vinelandii,导致细胞损伤和固的损失. 纳米粒子的毒性略高于微粒子.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 材料科学 材料科学 材料科学
背景情况:
- 二氧化瓦纳 (VO2) 具有独特的相位过渡特性,导致各种应用.
- VO2是一种潜在的重金属污染物,需要进行环境风险评估.
- 生物固化对全球循环至关重要,它是由像Azotobacter vinelandii这样的细菌介导的.
研究的目的:
- 研究VO2微/纳米颗粒对固定细菌Azotobacter vinelandii的环境生物效应.
- 评估毒性机制和对固活动的影响.
- 为了比较VO2微粒与纳米颗粒的毒性.
主要方法:
- 暴露Azotobacter vinelandii在不同度的VO2微粒/纳米颗粒 (10和30毫克/升) 中.
- 细胞损伤的评估 (细胞亡,氧化应激,身体损伤,基因毒性).
- 分析与应激反应和固定相关的基因表达 (nif 和 vnf 基因).
- 测量乙降解活性以确定固定效率.
主要成果:
- 10和30毫克/升的VO2诱导了A. vinelandii的显著危险,包括细胞亡,氧化损伤,基因毒性和固定损失.
- 在A. vinelandii中,上调基因与应激反应有关,而下调基因与能量代谢有关.
- 在10 mg/L,VO2降低了nif基因表达,但增加了vnf基因表达,增强了无氧乙烯的减少.
- 在测试条件下,VO2纳米粒子的毒性略高于微粒子.
结论:
- VO2对固细菌构成环境风险,影响细胞完整性和功能.
- 细菌的反应涉及应激适应和改变固定通路.
- VO2纳米颗粒可能比微粒带来更大的环境危害.
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