植物招募杀虫细菌来抵御草食动物的攻击
Wenyu Xu1, Xiaoxiao Sun1, Liang Mi2
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
植物可以招募杀虫细菌来防御害虫. 被幼虫感染的花生植物丰富了特定的Enterobacter细菌,保护它们免受进一步的草食动物伤害.
科学领域:
- 微生物生态学 微生物生态学
- 植物与昆虫的相互作用
- 细菌遗传学 细菌遗传学
背景情况:
- 害虫的食改变了树枝细菌,激活了植物的防御通路 (酸,莉酸).
- 目前尚不清楚植物是否积极招募特定的杀虫剂微生物来对抗草食动物.
研究的目的:
- 为了调查花生是否丰富杀虫细菌,以应对草食动物的食.
- 为了确定这些丰富的细菌是否增强了植物对害虫的抵抗力.
主要方法:
- 高通量测序16S rRNA基因安普利康,以分析树根球细菌社区的变化.
- 对根球细菌进行针对 Holotrichia parallela 幼虫的隔离和毒性测试.
- 用绿色光蛋白 (GFP) 标记的细菌进行分裂根系实验.
主要成果:
- 幼虫感染在24小时内迅速改变了树根球细菌结构,丰富了Enterobacteriaceae (Enterobacter).
- 杆菌在感染后形成了一个更复杂的共同发生网络.
- 四种细菌分离物显示出对H. parallela幼虫的高毒性;Enterobacter sp. IPPBiotE33富含未被吃掉的根,并减轻了草食动物的损害.
结论:
- 草食动物的入侵可以诱导植物组装具有特定幼虫杀虫活性的细菌.
- 丰富的肠道细菌 sp. IPPBiotE33增强了花生对H. parallela的抵抗力,这表明植物介导的微生物防御策略.
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