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创新的方法来抑制分子印记聚合物中的非特异性吸附,用于传感应用

Abdelhafid Karrat¹, Aziz Amine¹

¹Laboratory of Process Engineering and Environment, Faculty of Sciences and Techniques, Hassan II University of Casablanca, B.P. 146 Mohammedia, Morocco.

Biosensors & bioelectronics

|January 24, 2024

概括

用SDS和CTAB等表面活性剂对分子印记聚合物 (MIP) 的静电修饰有效地消除了非特异性结合. 这增强了MIPs.

科学领域:

聚合物化学聚合物化学
分析化学分析化学
材料科学材料科学材料科学

背景情况:

分子印记聚合物 (MIP) 模仿抗体进行选择性分子识别.
在MIP腔外的非特异性结合限制了它们的传感性能.
表面活性剂的修改为改善MIP选择性提供了一个潜在的解决方案.

研究的目的:

为了提高MIP的选择性和性能,用于硫甲醇 (SMX) 检测.
调查使用二甲基硫酸盐 (SDS) 和 cetyl trimethyl ammonium bromide (CTAB) 来进行MIP修改.
为了评估静电修饰对MIP非特异性吸附的影响.

主要方法:

合成的MIP和非印刷聚合物 (NIP).
使用SDS和CTAB表面活性剂修改的MIP.
分析了SMX的结合同热量和动力吸附.
进行稳定性研究,并将该方法应用于真实样本 (牛奶,水).

主要成果:

最低产品价格显示出比NIP更高的SMX吸附能力.
修改SDS和CTAB有效消除了MIP中的非特异性吸附.
开发的MIP+-SDS/CTAB显示选择性SMX吸附,检测极限较低 (6 ng/mL与MIP+-SDS).
修改后的MIP显示出良好的稳定性,并成功应用于乳和水样中的SMX检测,有望恢复.

结论:

用表面活性剂对MIP进行静电修改是一种可行的策略,可以提高选择性并减少非特异性结合.
SDS和CTAB是有效的表面活性剂,可以提高分子识别中的MIP性能.
开发的方法显示了在复杂矩阵中对SMX的敏感和选择性现场检测的潜力.

关键词:

有分子印记的聚合物.非特定的吸附吸附.智能手机检测检测智能手机检测硫甲佐 (sulfamethoxazole) 是一种硫甲佐 (sulfamethoxazole) 的一种化合物.表面活性剂是一种表面活性剂.

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