除了抗生素耐药性: whiB7转录因子协调了适应性反应,以对氨酸饥饿的真菌细菌
Nicholas C Poulton1, Michael A DeJesus1, Vanisha Munsamy-Govender1
1Laboratory of Host-Pathogen Biology, The Rockefeller University, New York, NY, USA.
Cell chemical biology
|January 24, 2024
概括
这种细菌是Mycobacteria.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 致病性菌根细菌导致全球显著的发病率和死亡率.
- 菌根菌中的 whiB7 应激反应通过激活内在耐药机制来赋予抗生素耐药性.
- 诱导 whiB7 表达的信号尚未完全理解,这阻碍了治疗策略.
研究的目的:
- 为了确定调节菌体中 whiB7 表达的基因.
- 阐明控制whiB7应激反应的信号通路.
- 了解 whiB7.7 的代谢作用和进化保存.
主要方法:
- 使用了一个基于记者的全基因组CRISPRi表皮质检查屏幕.
- 研究了5'监管上游开放阅读框架 (uORF) 在 whiB7 监管中的作用.
- 研究了 whiB7 与氨酸生物合成酶aspC.C.之间的相互作用.
主要成果:
- 一个屏幕识别了150个真菌细菌基因,当被抑制时,导致构成性 whiB7 表达.
- WhB7的表达受其5'调节性uORF的氨基酸组成的调节,使其能够感知氨基酸饥饿.
- 具体来说,WhB7通过与aspC的反循环协调了对氨酸饥饿的适应性反应.
结论:
- WhB7 作为一种代谢调节剂,感知氨基酸饥饿.
- 这些发现揭示了 whiB7 和氨酸生物合成之间的新反循环.
- 这项研究提供了关于whiB7在不同菌根菌种中的进化保存的见解.
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