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尿源干细胞的年龄相关能量代谢和转录组变化

Elisabete Ferreiro¹, Mariana Monteiro², Francisco Pereira³

¹CNC-Center for Neuroscience and Cell Biology, University of Coimbra, UC Biotech, Cantanhede, Portugal; Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra 3000-548, Portugal.

Mechanisms of ageing and development

|January 24, 2024

概括

年长的妇女老年妇女.

科学领域:

老年学和再生医学的研究.
细胞生物能量学细胞生物能量学
非侵入性的生物标志物

背景情况:

全球60岁以上的人口迅速增加,需要对健康衰老和与年龄有关的疾病进行研究.
评估与年龄有关的变化的非侵入性方法对于开发有效的干预措施至关重要.
尿源干细胞 (UDSCs) 为衰老研究提供了一个有希望的,易于获得的细胞来源,但它们在不同年龄段的生物能特征仍未得到充分探索.

研究的目的:

研究来自年轻和老年妇女的尿源干细胞 (UDSC) 的生物能特性和转录特征.
确定UDSC是否可以作为研究与年龄相关的细胞变化的非侵入性模型.
在UDSC中识别潜在的与年龄相关的生物标志物.

主要方法:

从年轻 (22-35岁) 和老年 (70-94岁) 成年女性中隔离和培养UDSC.
使用氧气消耗率 (OCR) 评估细胞生物能量,以确定最大氧气消耗和生物能量健康指数.
RNA测序 (RNAseq) 用于分析UDSC中的与年龄相关的转录差异.
探索性数据分析用于特征选择和UDSC分类的应用.

主要成果:

与年轻女性相比,来自老年妇女的UDSC显示出显著更高的最大氧气消耗和生物能健康指数.
转录分析揭示了参与葡萄糖化和雌激素代谢的基因的下调,以及来自老年捐赠者的UDSCs中的炎症相关基因的上调.
意想不到的是,老年UDSC中改善的细胞生物能学与与年龄相似的转录组签名相吻合.

结论:

UDSCs显示出不同的生物能量和转录组概况,这些概况在年轻人和老年人之间有所不同.
老年UDSCs表现出增强的生物能量能力,但具有衰老的转录组,表明与年龄相关的复杂适应.
UDSCs为推进衰老研究和探索与年龄相关疾病的潜在治疗策略提供了有价值的非侵入性模型.

关键词:

衰老的衰老衰老的衰老机器学习是机器学习.代谢过程中的代谢.线粒体中的线粒体.转录组转录组就是一个转录组.从尿液中获得的干细胞.女人女人女人女人女人

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