相关实验视频

Updated: Jul 5, 2025

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

开发基于工程外体和瘤细胞溶解物的新型HPV治疗疫苗构造

Fatemeh Rezaei¹, Azam Bolhassani¹, Seyed Mehdi Sadat¹

¹Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.

|January 24, 2024

概括

携带Hsp27-E7融合蛋白的工程外体 (Exo) 显示出作为治疗性人类乳头瘤病毒 (HPV) 疫苗的前景,在小鼠中显示出强大的抗瘤效应和免疫反应.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
疫苗开发疫苗开发

背景情况:

人类乳头瘤病毒 (HPV) 感染是一个全球性的健康问题,需要除了目前的预防性疫苗之外进行治疗干预.
现有的HPV病变和恶性瘤需要新的治疗策略,强调需要有效的治疗疫苗.

研究的目的:

评估三个不同的治疗性HPV疫苗候选人的免疫刺激和抗瘤功效.
为了比较重组蛋白,瘤细胞溶解物 (TCL) 和工程外体 (Exo) 平台的效果,所有这些都包含了Hsp27-E7融合结构.

主要方法:

使用大肠杆菌表达系统生产重组Hsp27-E7蛋白质.
通过透视病毒系统生成基于TCL和EXO的疫苗结构 (Hsp27-E7).
在预防和治疗小鼠模型中评估免疫和抗瘤效应.

主要成果:

这三种疫苗方案 (重组Hsp27-E7,TCL-Hsp27-E7,Exo-Hsp27-E7) 都诱导了显著的抗瘤免疫反应,具有相同的75%的无瘤存活率.
Exo-Hsp27-E7表现出优异的Granzyme B分泌,表明增强的细胞毒性T淋巴细胞 (CTL) 活性.
疫苗通常促进Th1和CTL反应,从而在小鼠模型中产生有效的抗瘤活性.

结论:

工程外体 (Exo-Hsp27-E7) 候选疫苗是最有前途的策略,因为它具有更高的Granzyme B水平,减少了瘤生长,并改善了安全性.
这种基于外体的治疗疫苗具有治疗现有的HPV相关疾病的潜力.

关键词:

基于外体细胞的工程疫苗.热冲击蛋白 27 热冲击蛋白 27 人类乳头瘤病毒人类乳头瘤病毒基于蛋白质的疫苗疫苗基于瘤细胞溶解酸的疫苗

更多相关视频

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo

Published on: September 28, 2022

相关概念视频

Cancer Vaccines

Cancer Vaccines

Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.