香味受体识别坎佛在坎佛树专家Orthaga achatina (Lepidoptera:Pyralidae) 的香味受体
Yu Ma1, Ting-Ting Yang1, Shuang Ni1
1Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Journal of agricultural and food chemistry
|January 24, 2024
概括
蝶Orthaga achatina被坎佛吸引,坎佛是一种以前作为驱逐剂使用的化学物质. 研究人员确定了一种特定的嗅觉受体,OachOR16,对于检测坎佛至关重要,为害虫控制提供了新的途径.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
- 化学生态化学生态学
背景情况:
- 坎佛是历史上的驱虫剂,但 *Orthaga achatina* 幼虫在坎佛树 (*Cinnamomum camphora*) 上食.
- 在O. achatina*中,珀感知的行为和分子机制以前是未知的.
研究的目的:
- 为了研究O. achatina*成年人对坎的行为反应.
- 为了确定O. achatina*中珀感知的分子基础.
主要方法:
- 进行了行为测试,以评估成年人对花的吸引力.
- 分析了天线转录组以确定嗅觉受体 (OR) 基因.
- 使用Xenopus卵细胞表达系统来测试OR对坎佛的反应.
- 用分子对接和位点定向突变发生来确定关键的结合残留物.
主要成果:
- 男性和女性 *O. achatina* 成人表现出对坎佛的行为吸引力.
- 只有OachOR16/Orco受体基因对坎有显著的反应.
- 在OachOR16中发现的Ser209残留物被认为对珀结合至关重要.
结论:
- *Orthaga achatina*已经适应使用坎佛作为宿主植物信号.
- 在O. achatina*中,OachOR16受体在探测中起着至关重要的作用.
- 这项研究为开发新型昆虫驱虫剂提供了一个分子标.
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