治疗效果和机制的Yougui Wan在老鼠与椎间盘退化
She Ma1,2, Kan Liu2, Jing-Yan Yang1,2
1Beijing University of Chinese Medicine, Beijing, 100029, China.
Journal of orthopaedic surgery and research
|January 24, 2024
概括
Yougui Wan通过减少炎症和细胞外基质降解来缓解大鼠的椎间盘退化. 这种传统中医药可能会调节Notch信号通路以达到其治疗效果.
科学领域:
- 生物医学科学 生物医学科学
- 药理学 药理学是指药理学的学科.
- 整形外科 整形外科 整形外科
背景情况:
- 椎间盘退化 (IDD) 是腰部疼痛的一个重要原因.
- 目前对IDD的治疗有局限性,需要探索新的治疗策略.
- 传统中医 (TCM) 为管理退行性疾病提供了潜在的途径.
研究的目的:
- 为了研究Yougui Wan的治疗机制,在腰椎间盘退化病的老鼠模型中.
- 评估Yougui Wan对IDD炎症标记物,细胞外矩阵组件和细胞亡的影响.
- 为了探索痕信号通道在Yougui Wan的行动中的潜在参与.
主要方法:
- 在Sprague-Dawley大鼠中通过环孔穿刺建立了一个椎间盘退化 (IDD) 模型.
- 在模拟后的两周内,老鼠通过输液接受了Yougui Wan的治疗.
- 使用ELISA测量IL-10,MIF和TNF-α的血清水平.
- 分析了原II和Notch1的蛋白质表达,并采用了西方涂抹方法.
- 使用TUNEL试验检测出细胞核中的亡.
主要成果:
- 与IDD模型组相比,Yougui Wan治疗显著增加了血清IL-10水平,同时降低了MIF和TNF-α水平.
- 服用Yougui Wan导致原II的蛋白质表达增加,并在椎间盘组织中减少Notch1的表达.
- 优基万治疗减少了细胞核中核细胞的亡.
结论:
- 尤吉万在抑制与IDD相关的炎症反应方面表现出潜力.
- 这种治疗似乎减轻了细胞外矩阵的降解,并减少了细胞核的细胞亡.
- 这些发现表明,Yougui Wan可能会缓解椎间盘退化,可能通过调节Notch信号通路.
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