识别,化学合成,和爬行动物甲格雷林受体结合的爬行动物甲格雷林
Hidekazu Katayama1, Hiroyuki Kaiya2,3,4
1Department of Bioengineering, School of Engineering, Tokai University, Hiratsuka, Japan.
概括
研究人员在日本甲鼠 (Gekko japonicus) 中鉴定出 ghrelin 激素及其受体. 他们开发了一种高效的草 ghrelin 的合成,证实了它的生物活性和在爬行动物中的存在.
科学领域:
- 进行比较的内分泌学.
- 分子生物学分子生物学
- 类激素研究研究 类激素研究
背景情况:
- 格林是一种脊椎动物的胃肠道类激素.
- 格林在Ser侧链中经历独特的八化.
- 爬行动物的格林的存在和功能在很大程度上仍未被探索.
研究的目的:
- 在日本 (Gekko japonicus) 中识别编码格林及其受体的基因.
- 为了建立一种高效的合成方法来制造甲 ghrelin,特别是用C端的histidine残留物.
- 为了确认合成的草 ghrelin 与其受体的功能.
主要方法:
- 格林和其受体在Gekko japonicus中的基因鉴定.
- 使用优化的固相合成策略,化学合成八基化格林.
- 在HEK 293细胞中表达甲 ghrelin受体.
- 在其受体上对合成草 ghrelin 的功能测定.
主要成果:
- 格林及其受体的基因在Gekko japonicus中成功被确定.
- 建立了一种高效的合成方法,以最小的副作用生产G. japonicus ghrelin.
- 合成的草 ghrelin 证明了与其表达的受体完全响应.
- 甲格林的C端残留物被确定为histidine.
结论:
- 格林系统,类似于哺乳动物的格林系统,存在于爬行动物子Gekko japonicus.
- 开发的合成方法是高效的,适用于合成来自其他物种的 ghrelin.
- 这项研究提供了关于脊椎动物林系统演变的见解.
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