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细胞毒性Citrobacter braakii从人类胃中分离出来的表征

Mengchao Yu¹, Fangyu Xie², Chengzhen Xu³

¹Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, University of Health and Rehabilitation Sciences, Qingdao, China.

|January 25, 2024

概括

杆菌 (Citrobacter braakii) 是一种格拉姆阴性细菌,与慢性胃炎和胃炎有关. 这项研究揭示了它对胃细胞的炎症和细胞毒性作用,这表明它在胃粘膜损伤中发挥了作用.

科学领域:

微生物学微生物学
胃肠病学胃肠病学
病变的发生和发病.

背景情况:

杆菌 (Citrobacter braakii) 是一种无氧的,在各种环境中发现的阴性细菌,与肠道,呼吸道和泌尿道的粘膜炎症有关.
C. braakii在胃粘膜病原体中的作用尚不清楚.
慢性胃炎 (CG) 影响了人口的很大一部分,鉴定导致的因素至关重要.

研究的目的:

调查C. braakii在慢性胃炎中的存在和作用.
为了阐明C. braakii在胃上皮细胞中的致病机制.

主要方法:

在患有慢性胃炎的患者中检测C. braakii.
隔离和C. braakii菌株P1的特征,包括尿酶活性和抗酸性评估.
分泌系统 (T1SS,T5aSS,T6SS) 的分析和粘合物的预测.
使用胃 (AGS) 和非胃 (HEp-2) 表皮细胞进行体外粘附和细胞毒性测定.
测量乳酸脱酶 (LDH) 的释放和炎症性细胞因子 (IL-8,TNF-α) 的表达.

主要成果:

在35.5%的慢性胃炎患者中检测到C. braakii,与炎症严重程度相关.
隔离的C. braakii P1菌株显示出尿酶活性和抗酸性.
菌株P1粘附于AGS细胞,导致显著的LDH释放和增加IL-8和TNF-α的表达.
与HEp-2细胞相比,这些影响在胃上皮细胞 (AGS) 中更为明显.

结论:

C. braakii对胃上皮细胞表现出促炎和细胞毒性作用.
这种细菌具有毒性因素,包括尿酶活性和粘附能力.
C. braakii可能参与胃粘膜炎症的诱导和进展,特别是慢性胃炎.

关键词:

在Citrobacter braakii中使用.粘附性粘附性粘附性粘附性细胞毒性细胞毒性基因组基因组是基因组的组成部分.介质蛋白-8-8的使用方法

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