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一项关于核心流细胞测量设施的调查:仪器维护,使用和融资

Sonal Priya¹, Kathleen Brundage², Ron Orlando¹

¹Department of Chemistry University of Georgia AthensGeorgia30602 USA.

Journal of biomolecular techniques : JBT

|January 25, 2024

概括

流细胞计共享资源实验室 (SRLs) 依赖于各种仪器. 这项调查揭示了这些核心设施内流动细胞计维护,使用和更换因素的洞察力.

科学领域:

生物技术是生物技术.
生命科学生命科学
研究基础设施研究基础设施

背景情况:

流细胞计对于免疫学,分子生物学和癌症生物学研究至关重要.
共享资源实验室 (SRL) 广泛使用流细胞计仪器.
不同的流量细胞计类型 (分析仪,分类器,成像,质量) 存在独特的维护挑战.

研究的目的:

为了调查仪器维护和流量细胞计SRLs的营业额.
了解影响流动细胞计正常运行时间,使用和更换的因素.
收集有关日常维护,深层清洁,质量控制和资金来源的数据.

主要方法:

进行了一项独立的在线调查.
从146个核心设施收集的数据.
问题集中在仪器正常运行时间,使用,维护,成本,清洁,质量控制,资金和更换原因上.

主要成果:

该调查提供了典型的SRL的操作概述.
答案反映了研究人员在流细胞计管理方面的实际经验.
收集了仪器性能和用户报告的问题关键数据.

结论:

了解维护和营业额对于SRL效率至关重要.
调查结果为管理流细胞计核设施提供了宝贵的见解.
这项研究强调了流量细胞计利用和维护的实际方面.

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相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...