乳制品来源的形成多药耐药性黄金葡萄球菌的生物膜:分子和进化观点
1Ph.D. Student in Veterinary Medicine, Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Iranian journal of veterinary research
|January 25, 2024
概括
牛乳腺炎的生物膜产生的金黄色葡萄球菌株表现出高的多药性耐药性. 对icaA基因的进化分析揭示了重组和积极选择,表明适应抗微生物压力.
科学领域:
- 微生物学 微生物学
- 进化生物学 进化生物学
- 遗传学 遗传学 是一个
背景情况:
- 黄金葡萄球菌 (Staphylococcus aureus) 生产生物膜是多药性耐药性的一个主要驱动因素.
- 人们对S. aureus的进化适应机制和病原性仍然不了解.
研究的目的:
- 为了研究S. aureus从牛亚临床乳腺炎中分离出来的生物膜形成潜力.
- 分析相关的多药耐药性模式.
- 在这些隔离物中进行icaA基因的进化分析.
主要方法:
- 使用刚果红色 (CRA) 和微位板 (MTP) 方法来检测生物膜.
- 用于icaA基因和Kirby-Bauer盘扩散进行抗生素耐药性分析的PCR.
- 使用生物信息学工具对icaA基因进行了分子和进化分析.
主要成果:
- 63.93%的S. aureus分离物中含有icaA基因;CRA比MTP更有效地检测了生物膜.
- 生物膜阳性分离物显示出高比率的甲素耐药黄金色细菌 (MRSA) (78.21%) 和抗胺素耐药黄金色细菌 (VRSA) (56.41%).
- 所有的分离物 (100%) 都具有多重耐药性;进化分析表明了codon偏差,重组事件和icaA基因的积极选择.
结论:
- 具有生物膜阳性的金黄色细菌分离体显示出对甲基西林,万科米和多种抗生素具有强烈的耐药性.
- 在 icaA 基因中的 Codon 偏差很可能是由于突变和选择.
- 重组和积极选择事件表明细菌适应抗微生物药物和宿主免疫逃避的策略.
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