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Yadong Xue¹, Tingting Tong¹, Yuyao Zhang²

¹Department of Pharmacology (The Key Laboratory of Cardiovascular Research, Ministry of Education, State Key Laboratory of Frigid Zone Cardiovascular Diseases, Ministry of Science and Technology) at College of Pharmacy, Harbin Medical University, Harbin, China.

Frontiers in pharmacology

|January 25, 2024

概括

棕酸通过增加TRPM4表达来诱导血管内皮损伤,导致亡. 向TRPM4或miR-133a-3p可以预防内皮损伤和动脉样硬化.

科学领域:

心血管研究研究心血管研究
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

血管内皮损伤有助于动脉样硬化和心血管疾病.
棕酸 (PA) 促进内皮细胞亡和动脉样硬化.
暂时受体潜在的 Melastatin 4 (TRPM4) 通道与内皮功能障碍有关,但它们在动脉样硬化中的特定作用需要阐明.

研究的目的:

研究TRPM4在棕酸诱导的血管内皮损伤中的作用和机制.
在内皮功能障碍的背景下探索miR-133a-3p和TRPM4之间的关系.

主要方法:

在PA治疗后评估TRPM4,亡和炎症标志物.
利用TRPM4淘汰模式,测量了细胞内Ca2+,线粒体膜潜力和活性氧物种.
通过双 luciferase 记者测定证实了 miR-133a-3p 与 TRPM4 的结合.

主要成果:

治疗PA上调TRPM4,导致过载,线粒体损伤和内皮亡.
TRPM4上调导致IL-1β,ICAM-1和VCAM1.1的增加.
miR-133a-3p直接准TRPM4mRNA,抑制其表达;减少miR-133a-3p加剧了PA诱导的损伤,而其过度表达减弱了它.

结论:

在棕酸诱导的血管内皮损伤中,TRPM4起着关键作用.
向TRPM4或调节miR-133a-3p水平为预防内皮损伤和动脉样硬化提供了潜在的治疗策略.

关键词:

在 MiR-133a-3p 里面.TRPM4 TRPM4 在线播放过高脂血症是什么意思棕酸是一种棕酸.血管内皮损伤血管内皮损伤

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