中性的合成和细胞吸收 (((I) 吗啡林复合体
Christie A Bader1, Peter V Simpson2, Elena Dallerba2
1Clinical and Health Science, University of South Australia, Adelaide, South Australia 5000, Australia. sally.plush@unisa.edu.au.
Dalton transactions (Cambridge, England : 2003)
|January 25, 2024
概括
新的以为基础的光探测器与摩尔福林准部分为溶酶体成像提供了优越的替代方案. 这些探针在活细胞中提供明亮,光稳定的成像,克服了传统有机光剂的局限性.
科学领域:
- 无机化学 无机化学
- 生物成像是一种生物成像.
- 细胞生物学 细胞生物学
背景情况:
- 光成像中的 lysosome 向是常见的.
- 有机成像剂经常遭受小斯托克斯转移,短寿命和光漂白.
- 基于的光平台提供了更好的光稳定性和发射寿命.
研究的目的:
- 开发基于的新型光成像剂用于 lysosomes.
- 为了克服传统有机光探针的局限性.
- 评估这些新药物在活细胞成像中的有效性和安全性.
主要方法:
- 合成复合物与用形态素替代的连接物.
- 对光物理性质的评估 (斯托克斯转移,辐射寿命,光稳定性).
- 使用共聚焦光显微镜在活细胞中进行细胞吸收和毒性研究.
主要成果:
- 合成了三种含有形态素连接体的复合体.
- 两个复合体在低度 (20μM) 的细胞中显示出明亮的辐射.
- 这些复合物在高达100μM的度下是无毒的,适合活细胞成像.
结论:
- 以为基础的光探针与形态素准是有效的溶酶体成像.
- 这些探针比传统的有机光剂具有优势.
- 开发的药物适用于活细胞内体-溶解体的共聚焦光成像.
相关概念视频
02:52Stereoisomerism
11.9K
Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula.
Transition metal complexes often exist as geometric isomers, in which the same atoms are connected through the same types of bonds but with differences in their orientation in space. Coordination complexes with two different ligands in the cis and trans positions from a ligand of interest form isomers. For example, the octahedral [Co(NH3)4Cl2]+ ion has two isomers (Figure 1) In the cis...
Isomers are different chemical species that have the same chemical formula.
Transition metal complexes often exist as geometric isomers, in which the same atoms are connected through the same types of bonds but with differences in their orientation in space. Coordination complexes with two different ligands in the cis and trans positions from a ligand of interest form isomers. For example, the octahedral [Co(NH3)4Cl2]+ ion has two isomers (Figure 1) In the cis...
11.9K
02:34Structural Isomerism
19.2K
Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
19.2K
01:19Phase II Reactions: Miscellaneous Conjugation Reactions
62
Phase II biotransformations are detoxification mechanisms that conjugate xenobiotics with endogenous substances, neutralizing their toxicity.
A key example involves the conjugation of cyanide ions, which impair cellular respiration and alter hemoglobin into non-oxygen-carrying cyanmethemoglobin. To neutralize this threat, a sulfur atom from thiosulphate is transferred to the cyanide ion, catalyzed by the enzyme rhodanese, resulting in an inactive compound called thiocyanate. The production of...
A key example involves the conjugation of cyanide ions, which impair cellular respiration and alter hemoglobin into non-oxygen-carrying cyanmethemoglobin. To neutralize this threat, a sulfur atom from thiosulphate is transferred to the cyanide ion, catalyzed by the enzyme rhodanese, resulting in an inactive compound called thiocyanate. The production of...
62