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在小鼠棕色脂肪细胞中介素-6表达的基础是调节机制

Xiajie Fu¹, Masaru Murakami², Osamu Hashimoto³

¹Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.

Cell biochemistry and function

|January 25, 2024

概括

棕色脂肪细胞分泌IL-6 (IL-6),这是一个关键的阿迪波金. 这项研究表明,cAMP依赖蛋白激酶 (PKA) 途径和其他信号途径一起,调节激活的棕色脂肪细胞中的IL-6表达.

科学领域:

细胞生物学细胞生物学
代谢过程中的代谢.
内分泌学在内分泌学.

背景情况:

脂肪细胞,包括白色,棕色和色类型,对于调节系统能量平衡至关重要.
脂肪细胞产生生物活性分子称为adipokines,与白色脂肪细胞相比,棕色脂肪细胞 (batokines) 的生物活性分子知之甚少.

研究的目的:

研究控制棕色脂肪细胞中介质素-6 (IL-6) 的表达的调节机制.
通过激活的棕色脂肪细胞识别参与IL-6生产的信号通路.

主要方法:

利用了初级棕色脂肪细胞和HB2棕色脂肪细胞细胞系.
用上腺素,β-上腺素受体激动剂和福斯科林 (Fsk) 刺激细胞来激活cAMP依赖的蛋白激酶 (PKA) 途径.
采用西式涂抹来评估蛋白质酸化 (CREB,p38 MAP 激酶,STAT3) 和表达 (C/EBPβ).
使用途径抑制剂来确定PKA,p38 MAP激酶,STAT3和C/EBPβ途径对IL-6表达的必要性.
研究了维生素C对Fsk诱导的IL-6表达通过细胞外信号调节激酶 (ERK) 活性的影响.

主要成果:

上腺素和β-上腺激动剂增加了棕色脂肪细胞中的IL-6表达.
福斯科林 (Fsk) 治疗显著刺激了棕色脂肪细胞和肌管中的IL-6表达.
Fsk迅速增加了酸化的CREB和p38 MAP激酶.
长时间的Fsk治疗增加了STAT3酸化和C/EBPβ表达.
由Fsk诱导的最大IL-6表达需要PKA,p38 MAP激酶,STAT3和C/EBPβ通路.
维生素C通过ERK激活增强了Fsk诱导的IL-6表达.

结论:

PKA,p38 MAP 激酶,STAT3 和 C/EBPβ 信号级联对于调节激活的棕色脂肪细胞中的IL-6表达至关重要.
维生素C可以通过ERK通路调节棕色脂肪细胞中IL-6的产生.
这项研究为棕色脂肪组织中关键的巴托卡因IL-6的调节提供了基础的见解.

关键词:

棕色脂肪细胞是什么福斯科林是一种福斯科林.互乐金-6是其中的一种.转录转录是一种转录.维生素C是维生素C的重要组成部分.

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