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巨细胞调节糖尿病伤口中与愈合相关的纤维细胞

Yu Xiao^1,2, Jieqi Qian³, Xiaohui Deng^1,4

¹Department of Endocrinology and Metabolism, The First Affiliated Hospital of Nanchang University, 17 Yongwaizheng Street, Nanchang, 330006, China.

Molecular biology reports

|January 25, 2024

概括

糖尿病的伤口愈合受到改变的巨细胞和纤维细胞的影响. 在糖尿病巨细胞中,介质素-6 (IL6) 治疗可以恢复纤维细胞功能并改善愈合,为糖尿病足提供潜在的治疗方法.

科学领域:

细胞生物学细胞生物学
伤口治愈研究研究伤口治愈研究
糖尿病并发症糖尿病并发症

背景情况:

糖尿病足表现出受损的微环境,影响炎症,血管生成和纤维化.
巨细胞和纤维细胞在糖尿病伤口中表现出改变的表型.
一个特定的纤维细胞亚群 (高MMP1,MMP3,MMP11,CHI3L1) 与糖尿病伤口的成功愈合有关,但巨细胞调节是未知的.

研究的目的:

研究巨细胞在调节糖尿病伤口愈合中与愈合相关的纤维细胞中的作用.
探索IL6作为糖尿病中巨细胞功能缺陷的治疗剂的潜力.

主要方法:

对公共皮肤数据库的生物信息分析,以确定糖尿病改变的基因.
利用小鼠模型进行糖尿病伤口愈合.
进行了糖尿病和非糖尿病巨细胞和纤维细胞的共同培养实验.
评估了MMP1,MMP3,MMP11,CHI3L1和VEGF-A的基因表达.

主要成果:

糖尿病伤口显示了巨细胞中IL1β,IL6,IL10和VEGF-A的空间时间表达的改变.
在糖尿病伤口纤维细胞中观察到MMP1,MMP3,MMP11,CHI3L1和VEGF-A的表达受损.
糖尿病巨细胞减少了关键基因的纤维细胞表达;IL6补充恢复了这一点.
在糖尿病小鼠中,巨细胞特异性IL6表达增强了伤口愈合和血管生成.

结论:

巨细胞在激活与伤口愈合相关的纤维细胞中发挥作用.
糖尿病中缺陷的巨细胞可以通过IL6治疗来纠正.
IL6代表了糖尿病足病的有前途的治疗策略.

关键词:

糖尿病足部 (DFU) 是一种糖尿病的伤口 (DW)纤维细胞细胞.互白素-6 (IL6) 是一种蛋白质.巨细胞的两极分化巨细胞是一个巨细胞.

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