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生物模拟气溶暴露系统的流速和壁切应力特性
S Emma Sarles1, Edward C Hensel1, Janessa Terry1
1Department of Mechanical Engineering, Rochester Institute of Technology, 77 Lomb Memorial Drive, Rochester, NY 14623.
Journal of biomechanical engineering
|January 25, 2024
概括
研究人员开发了一种仿生气溶暴露系统 (BAES),以更好地模仿体外研究的肺吸入. 该系统提高了气溶输送精度和流动动力学,有助于肺损伤研究.
科学领域:
- 生物医学工程 生物医学工程
- 呼吸系统生理学 呼吸系统生理学
- 毒理学 毒理学 毒理学
背景情况:
- 目前的体外肺模型无法复制真实的吸入条件,阻碍实验结果与体内健康影响的准确相关性.
- 现有的系统往往缺乏仿生学,使用不准确的流速,并为细胞培养提供不充分的生物力学线索.
- 这限制了有效研究肺损伤和疾病进展的能力.
研究的目的:
- 设计和验证一种新的仿生气溶暴露系统 (BAES),该系统准确模拟人体呼吸道条件,用于体外气溶暴露.
- 解决当前体外系统在气溶沉积,流动动力学和生物机械线索方面的局限性.
- 为研究吸入气溶及其对肺部健康的影响提供一个更相关的平台.
主要方法:
- 生物模拟气溶暴露系统 (BAES) 的开发,包括电子烟适配器,口腔模块 (OCM) 和分叉暴露室 (BEC).
- 在过片的过渡过程中,气溶沉积分量的表征和挥发性损失.
- 在模拟吹气,吹气相关呼吸和潮吸入过程中测量空气流速.
- 在曝光室内的关键点评估墙壁剪切应力,特别是在分叉的上游和下游.
主要成果:
- 与传统设置 (0.116±0.021) 相比,BAES显示了减少的气溶沉积率 (0.098±0.015).
- 观察到的流量与吹气 (25毫升/秒),呼吸 (450毫升/秒) 和吸入 (350毫升/秒) 的编程值非常接近,最大流量为450毫升/秒.
- 墙壁剪切应力测量表明,条件超过了分叉的上游较低的目标,但低于下游的目标.
结论:
- 制造的BAES有效地解决了现有的体外气溶暴露系统中的关键缺口.
- 该系统为研究各种吸入的气溶及其对肺组织的影响提供了一个更加仿生平台.
- 该研究强调了 in silico 模型在将 in vitro 发现与 in vivo 条件相关联方面的实用性,补充了先进 in vitro 系统的发展.
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