相关实验视频

Updated: Jul 5, 2025

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

两个序列不相关的细菌因子干扰Arabidopsis WRKY54介导的免疫反应的分子基础

Haseong Kim¹, Jieun Kim^1,2, Du Seok Choi³

¹Plant Immunity Research Center, Seoul National University, Seoul, 08826, Republic of Korea.

The Plant journal : for cell and molecular biology

|January 25, 2024

概括

病原体效应者AvrRps4和PopP2准了Arabidopsis WRKY54,以抑制植物的免疫力. 这种相互作用涉及WRKY54中的特定残留物,突出了植物病原体免疫逃避的新机制.

科学领域:

植物免疫力植物免疫力
分子植物病原体相互作用
植物生物化学植物生物化学

背景情况:

阿拉比多普西斯塔利亚纳 WRKY 蛋白质在植物防御中至关重要,并且可以被病原体效应者的目标.
核酸结合和富含白素的重复 (NLR) 免疫受体RRS1 (AtWRKY52) 通过其WRKY域与作用器AvrRps4和PopP2相互作用.
效应因子选择性向WRKY蛋白质致病的确切机制在很大程度上是未知的.

研究的目的:

为了研究病原体作用因子AvrRps4和PopP2以及Arabidopsis WRKY蛋白之间的特定相互作用.
为了阐明这些效应器如何通过准WRKY蛋白来操纵植物免疫反应.
确定这些相互作用的关键分子决定因素及其在免疫抑制中的作用.

主要方法:

在Nicotiana benthamiana中进行过渡表达测试,以评估AtWRKYs的细胞死亡诱导.
同免疫沉试验证实了效应因子和WRKY蛋白之间的直接相互作用.
位点定向突变发生,以确定参与效应体结合和免疫抑制的关键氨基酸残留物.

主要成果:

一些IIIb亚组AtWRKY蛋白被确定为AvrRps4和PopP2.2的目标.
AtWRKY54诱导细胞死亡,并与AvrRps4和PopP2.2直接相互作用.
AvrRps4 和 PopP2 特别抑制了 AtWRKY54 诱导的细胞死亡,效应因子中的关键残留物调解了这种抑制.
在AtWRKY54中,一种特定的残留物 (E164) 对于与AvrRps4相互作用和诱导细胞死亡至关重要,其突变导致了逃避受效器介导的抑制.

结论:

AvrRps4和PopP2特别针对AtWRKY54,这是植物免疫系统的关键组成部分.
在AvrRps4/PopP2和AtWRKY54之间的相互作用对于病原体毒性和免疫逃避至关重要.
效应器和AtWRKY54中的特定残留物决定了这种相互作用的结果,为NLR受体功能和病原体策略提供了洞察力.

关键词:

阿拉比多普西斯 WRKY家族在WRKY54上在 AvrRps4 中使用.PopP2 PopP2 PopP2 PopP2 PopP2 PopP2 PopP2 PopP2 PopP2 PopP2 PopP2 PopP2 PopP2 Pop RRS1 - RRS1 - RRS1 - RRS1 - RRS1 - RRS1 - RRS1 - RRS1 - RRS1 - RRS1 - RRS1 - RRS1 - RRS1 - RRS1 - RRS1 - RRS1 - RRS2 - RRS2 - RRS3 - RRS4 - RRS4 - RRS4 - RRS5 - RRS5 - RRS6 - RRS7 - RRS8 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 - RRS9 -

更多相关视频

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Published on: May 21, 2019

Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

相关概念视频

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Defenses Against Pathogens and Herbivores

Defenses Against Pathogens and Herbivores

Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.

Plant Cell Wall

Plant Cell Wall

The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.