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剪接因子SRSF1对于在小鼠中寻找前体精子干细胞至关重要

Longjie Sun¹, Zheng Lv¹, Xuexue Chen¹

¹State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University, Beijing, China.

|January 25, 2024

概括

SRSF1对于小鼠精子干细胞 (SSC) 的生存和男性生育能力至关重要. 它的删除会损害SSC的定位,并导致不孕症,揭示SRSF1.

科学领域:

生殖生物学生殖生物学
分子遗传学分子遗传学
细胞生物学细胞生物学

背景情况:

精子干细胞 (SSC) 对于持续的精子生成和男性生育能力至关重要.
在小鼠SSC中替代拼接 (AS) 的作用仍然在很大程度上未被描述.
SRSF1是一种关键的剪接因子,对生殖细胞发育有潜在的影响.

研究的目的:

为了研究SRSF1在小鼠精子干细胞中的功能.
阐明SRSF1调节SSC中的基因表达和拼接的机制.
了解SRSF1删除对男性生育能力和SSC定位的影响.

主要方法:

交叉链接免疫沉降和测序 (CLIP-seq) 以识别与SRSF1结合的基因.
在胚胎细胞中缺乏SRSF1的条件淘汰 (cKO) 小鼠的生成.
全山染色,基因表达分析,多组学,免疫沉质谱和共同免疫沉.

主要成果:

SRSF1结合了许多与精子相关的基因,包括Plzf,Id4和Stra8.8.
对SRSF1的精子生殖特异性删除会导致男性不孕症和仅存在塞尔托利细胞综合征.
SRSF1通过AS调节Tiale1/Tiar表达,影响精子存率,并与SART1和RBM15.5等拼接因子相互作用.

结论:

SRSF1在精子的生存中起着至关重要的作用.
损坏的SRSF1功能会破坏SSC的指导,并导致男性不孕.
这些发现凸显了SRSF1在管理SSC发展的转录后监管网络中的重要性.

关键词:

在SRSF1中,我们可以使用SRSF1.只有塞尔托利细胞综合征.另一个替代拼接方法是拼接.细胞生物学细胞生物学发育生物学发展生物学对前体SSC的定位.不孕不育不孕不育不孕不育这里是鼠标鼠标鼠标鼠标鼠标鼠标.精子前体精子干细胞的前体.

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