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基因损失和cis-regulatory新性塑造了蜜蜂类酵母Hanseniaspora uvarum中的核心组织基因进化
Max A B Haase1,2, Jacob L Steenwyk3, Jef D Boeke1
1Institute for Systems Genetics and Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, 435 E 30th St, New York, NY 10016, USA.
Genetics
|January 25, 2024
概括
核心组织素基因调节在芽酵母中很古老,主要使用Spt10. 一种新的Mcm1介导调节在汉斯基亚斯波拉中出现,解基和DNA合成.
科学领域:
- 进化生物学是进化的生物学.
- 分子遗传学 分子遗传学
- 酵母基因组学 酵母基因组学
背景情况:
- 核心组织基因对DNA包装至关重要,并表现出保存的蛋白质序列,但不同的调节机制.
- 核心组织基因调控变化的进化动态和功能意义在很大程度上仍未被探索.
研究的目的:
- 为了研究4亿多年来在芽酵母中核心组织基因调节的进化历史.
- 识别和描述新的调节模式及其对细胞过程的影响.
主要方法:
- 对各种酵母菌种的核心基因组基因进行比较基因组学分析.
- 遗传学重建以推断调节模式出现的时间.
- 在转基因酵母 (Hanseniaspora uvarum) 中对调控元素和基因表达的功能分析.
主要成果:
- 正规的Spt10介导的核心组织基因调节是古老的,建立在3200-38000万年前之间,并保留在大多数现存物种中.
- 一种新的Mcm1介导的调节模式在快速发展的汉斯基亚斯波拉系中出现,通过cis调节变化取代了祖先的Spt10模式.
- 汉斯尼亚斯波拉的这种分歧与失去一个类似的基因组基因拷贝以及基因组和DNA合成的脱相吻合,尽管细胞分裂迅速.
结论:
- 核心组织基因调节是在酵母进化的早期建立的,但在特定的血统中经历了显著的分歧,如汉斯尼亚斯波拉.
- 替代性调节策略的出现,例如汉斯基亚体中的Mcm1介导模式,突显了基因调节在应对进化压力的适应性.
- 在H. uvarum中,组织蛋白和DNA合成之间的调节合的丧失表明,H. uvarum具有快速细胞循环的独特适应性.
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