聚乙烯纳米塑料通过调节PIWI相互作用RNA表达特征来诱导血管狭窄
Min Zhang1, Jun Shi2, Yiqian Zhu3
1Division of Cardiology, Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 200336, China.
Environmental pollution (Barking, Essex : 1987)
|January 25, 2024
概括
纳米塑料 (NP) 引起血管毒性,改变脂质代谢和动脉壁. 这项研究揭示了NP暴露后小鼠动脉PIWI相互作用RNA (piRNA) 水平的变化.
科学领域:
- 环境毒理学环境毒理学
- 血管生物学 血管生物学
- 分子生物学分子生物学
背景情况:
- 纳米塑料 (NP) 是普遍存在的环境污染物,对毒理问题越来越关注.
- 由于其小小的尺寸,NP比微塑料带来更大的风险,可能会影响各种组织.
- 对NP暴露对血管狭窄和相关分子机制的影响尚未得到充分理解.
研究的目的:
- 在小鼠动脉组织中研究聚乙烯纳米塑料 (PS-NP) 的血管毒性.
- 为了识别PS-NP暴露引起的分子变化,特别是PIWI相互作用RNA (piRNA) 变化.
- 阐明NP诱导的血管病理背后的调节机制.
主要方法:
- 对小鼠血管动脉组织暴露于红色光PS-NPs (100nm).
- 血液生化参数和病理组织学分析.
- 皮RNA的高通量测序,随后进行生物信息学和途径丰富分析 (GO,KEGG).
主要成果:
- PS-NPs被小鼠血管动脉组织有效地吸收.
- 暴露于PS-NP导致了血管毒性,其特征是脂质代谢变化和动脉壁加厚.
- 在暴露后的180天和30天,分别观察到1547和132个piRNAs (DEpiRNAs) 的显著差异表达.
- 丰富分析将DEpiRNA点基因与细胞生长,运动和MAPK信号通路联系起来.
结论:
- 这项研究提供了PS-NP暴露后小鼠血管动脉组织中piRNA水平变化的第一个证据.
- 暴露于PS-NP会诱导血管毒性,并调节piRNA的表达,影响关键的细胞过程.
- 这些发现有助于理解纳米塑料诱导的血管病理的分子机制.
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