纤维质矩阵因收缩的内含物而意外变软
Mainak Sarkar1, Brian M Burkel2, Suzanne M Ponik2
1Department of Mechanical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Acta biomaterialia
|January 25, 2024
概括
纤维基质中的多个收缩细胞可以意外地使周围环境变软,影响细胞行为. 这种由纤维曲引起的矩阵软化,挑战了细胞力量总是使矩阵变硬的想法.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 材料科学 材料科学 材料科学
背景情况:
- 细胞感知并响应其微环境的机械性质,特别是矩阵刚度.
- 众所周知,单个细胞的收缩会使纤维基质变硬,但对多个细胞的影响不太了解.
- 在细胞尺度上量化矩阵刚度是复杂的,因为非线性和异质纤维网络.
研究的目的:
- 通过计算模型和实验量化纤维质矩阵的刚度,具有多个同时收缩的包含.
- 为了研究集体细胞产生的力量如何改变细胞规模的矩阵力学.
- 为了确定细胞对由多个收缩细胞引起的改变矩阵性质的细胞反应.
主要方法:
- 利用计算建模模拟一个随机的纤维矩阵与多个收缩的包含.
- 进行实验以模仿纤维基体内的细胞收缩力.
- 使用计算模型量化矩阵刚度和分析纤维行为 (压缩,曲).
主要成果:
- 与预期相反,当被允许收缩以应对多重包含时,矩阵变软.
- 计算分析显示,矩阵软化是由矩阵纤维的轴向压缩和曲引起的.
- 这种曲引起的软化足以被细胞感知,改变了它们的形态和力生成.
结论:
- 局部化的细胞力量并不总是使纤维基质变硬;基质收缩可能导致软化.
- 细胞诱导的矩阵软化,由纤维曲驱动,影响细胞感知和行为.
- 这些发现为了解细胞矩阵机械相互作用及其在组织恒温和疾病中的作用提供了一个新的范式.
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