酸材料的相组合通过调节骨质结晶生成来影响骨形成
Paul Humbert1, Carina Kampleitner2, Julien De Lima1
1INSERM, UMR 1238, Phy-OS, Bone Sarcoma and Remodeling of Calcified Tissues, School of Medicine, University of Nantes, Nantes, France; INSERM, UMR 1229, RMeS, Regenerative Medicine and Skeleton, University of Nantes, Oniris, CHU Nantes, Nantes, France.
在酸 (CaP) 材料上的骨质细胞的形成,而不是介质细胞 stromal cell (MSC) 的存活,预测了骨再生. 早期的骨质细胞活性与骨形成相关,这表明它可以作为基于CaP的疗法中的预测标志物.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 细胞生物学 细胞生物学
背景情况:
- 在酸 (CaP) 生物陶上使用的人类介质干细胞 (hMSCs) 在骨组织工程中取得了临床成功.
- 之前的研究表明,骨质结晶生成之前的hMSC-CaP介导的骨形成.
- 卡帕相组合对骨质细胞反应和骨形成的影响尚不清楚.
研究的目的:
- 评估CaP生物陶相组合如何影响骨质细胞反应.
- 为了在体内模型中将骨质细胞反应与随后的骨形成相关联.
主要方法:
- 在实验室中对四种CaP生物陶的骨质细胞分化:酸 (HA),β-三酸 (β-TCP) 和两个双相复合物 (60/40和20/80 HA/β-TCP).
- 在裸体小鼠中用hMSCs植入CaP生物陶的子宫外植入.
- 评估体外骨质细胞形成,体内骨质细胞生成 (2周) 和骨形成 (8周).
主要成果:
- 所有的CaP材料,除纯净的HA外,在人体和小鼠前体中支持骨质细胞的形成.
- 在体内,在所有条件下都观察到显著的骨形成,相对数量:β-TCP>双相CaPs>HA.
- 早期骨质细胞形成 (2周) 与随后的骨形成有积极的相关性,而hMSC存活率没有.
结论:
- CaP生物陶的相组合影响骨质细胞形成和随后的骨再生.
- 早期的骨质结晶形成作为基于hMSC-CaP的骨再生结果的潜在预测标记.
- 了解骨质细胞生物材料相互作用对于开发用于骨再生的先进生物材料至关重要.
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