基因毒性和N-nitrosomorpholine活动的稳定性在UVA照射后
Haruna Mochizuki1, Yukari Nagazawa1, Sakae Arimoto-Kobayashi2
1School of Pharmaceutical Sciences, Okayama University, Japan.
Mutation research. Genetic toxicology and environmental mutagenesis
|January 25, 2024
概括
光激活的N-尼托索摩尔福林 (NMOR) 直接具有基因毒性,没有代谢激活. 被辐射的NMOR会导致突变和微核的形成,具有稳定的活性,构成潜在的环境健康风险.
科学领域:
- 环境毒理学环境毒理学
- 遗传毒性研究 研究遗传毒性研究
- 摄影化学的使用.
背景情况:
- 已知N-nitrosomorpholine (NMOR) 是一种环境污染物.
- NMOR的基因毒性通常需要代谢激活.
- 在没有激活的情况下,UVA照射对NMOR基因毒性的作用尚不清楚.
研究的目的:
- 为了研究UVA照射后的N-尼托索摩尔福林 (NMOR) 的基因毒性.
- 为了确定UVA激活的NMOR是否表现出无代谢激活的变异性和基因毒性.
- 评估光激活NMOR的稳定性和体内效应.
主要方法:
- 艾姆斯测试对UVA辐射NMOR的变异性.
- 在 HaCaT 细胞中进行体外微核 (MN) 形成试验.
- 在小鼠外周血液网状细胞中的体内MN形成试验.
- 在各种存储条件下评估NMOR活性稳定性.
主要成果:
- 在没有代谢激活的情况下,UVA照射的NMOR在Ames测试中显示出直接的致变性.
- 辐射NMOR诱导了HaCaT细胞和小鼠网状细胞中的微核形成.
- 照射NMOR的基因毒性活性至少在10天内保持稳定.
- 对于MN形成的作用频谱与NMOR的吸收曲线相关.
结论:
- 紫外线照射会诱导N-尼托索摩尔福林 (NMOR) 的直接基因毒性,而不依赖于代谢激活.
- 光激活的NMOR具有基因毒性风险,可能通过环境暴露途径.
- 需要进一步研究光激活NMOR对环境的影响.
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