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基于ETD的蛋白质定型改进了氨酸甲基化鉴定,并揭示了新型PRMT5基质

Lingzi Lu¹, Zilu Ye², Rou Zhang¹

¹School of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery, Sun Yat-sen University, Guangzhou 510006, China.

Journal of proteome research

|January 25, 2024

概括

这项研究引入了一种优化的质谱工作流程,以改善蛋白质氨酸甲基化检测. 这种新方法增强了对称二甲基氨酸 (sDMA) 位点的识别,并揭示了参与细胞应激反应的新基质.

科学领域:

生物化学生物化学
蛋白质组学是指蛋白质组学.
细胞生物学细胞生物学

背景情况:

蛋白质氨酸甲基化是真核生物中关键的翻译后修饰 (PTM).
由于中性损失,传统的质谱法难以确定甲基氨酸位点.

研究的目的:

开发一个优化的质谱工作流程,用于对氨酸甲基化进行深度分析.
改进对称二甲基氨酸 (sDMA) 的识别和局部定位.

主要方法:

开发了一种使用电子转移解离 (ETD) 和补充激活的优化质谱工作流程.
应用定量蛋白质组学来识别人类HeLa细胞中的SDMA位点.
在已识别的基质上进行生物化学研究,如SERBP1.

主要成果:

基于ETD的工作流显著提高了SDMA识别和站点定位.
确定了138个新的sDMA位点,表明潜在的PRMT5基质.
证明SERBP1甲基化对于其在氧化应激下招募到应力颗粒至关重要.

结论:

优化的工作流允许在人类细胞中广泛识别和定位sDMA位点.
发现了与sDMA相关的具有潜在显著生物功能的新型PRMT5基质.
突出阿金氨酸甲基化在调节细胞压力期间蛋白质局部化的重要性.

关键词:

在PRMT5中,氨酸甲基化的阿尔金因.电子转移解离,电子转移解离.蛋白质组学蛋白质组学

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